Cell Biology Antibodies 12

Anti-STK25 Antibody (CAB9726)

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SKU:
CAB9726
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-STK25 Antibody
Antibody SKU:CAB9726
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 297-426 of human STK25 (NP_006365.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:SW620, Mouse small intestine
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 297-426 of human STK25 (NP_006365.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EESS SEDS DIDG EAED GEQG PIWT FPPT IRPS PHSK LHKG TALH SSQK PAEP VKRQ PRSQ CLST LVRP VFGE LKEK HKQS GGSV GALE ELEN AFSL AEES CPGI SDKL MVHL VERV QRFS HNRN HLTS TR
Gene ID:10494
Uniprot:O00506
Cellular Location:Cytoplasm, Golgi apparatus
Calculated MW:37kDa/39kDa/48kDa
Observed MW:48kDa
Synonyms:STK25, SOK1, YSK1
Background:This gene encodes a member of the germinal centre kinase III (GCK III) subfamily of the sterile 20 superfamily of kinases. The encoded enzyme plays a role in serine-threonine liver kinase B1 (LKB1) signaling pathway to regulate neuronal polarization and morphology of the Golgi apparatus. The protein is translocated from the Golgi apparatus to the nucleus in response to chemical anoxia and plays a role in regulation of cell death. A pseudogene associated with this gene is located on chromosome 18. Multiple alternatively spliced transcript variants have been observed for this gene.
UniProt Protein Function:YSK1: Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
UniProt Protein Details:

Protein type:Protein kinase, STE; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; YSK subfamily

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm; Golgi apparatus

Molecular Function:protein binding; protein homodimerization activity; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: establishment of Golgi localization; Golgi localization; positive regulation of stress-activated MAPK cascade; protein amino acid autophosphorylation; protein amino acid phosphorylation; response to hydrogen peroxide; response to oxidative stress; signal transduction

NCBI Summary:This gene encodes a member of the germinal centre kinase III (GCK III) subfamily of the sterile 20 superfamily of kinases. The encoded enzyme plays a role in serine-threonine liver kinase B1 (LKB1) signaling pathway to regulate neuronal polarization and morphology of the Golgi apparatus. The protein is translocated from the Golgi apparatus to the nucleus in response to chemical anoxia and plays a role in regulation of cell death. A pseudogene associated with this gene is located on chromosome 18. Multiple alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]
UniProt Code:O00506
NCBI GenInfo Identifier:12643529
NCBI Gene ID:10494
NCBI Accession:O00506.1
UniProt Secondary Accession:O00506,Q15522, Q5BJF1, A8K6Z3, A8K7D2, B7Z9K1,
UniProt Related Accession:O00506
Molecular Weight:37,297 Da
NCBI Full Name:Serine/threonine-protein kinase 25
NCBI Synonym Full Names:serine/threonine kinase 25
NCBI Official Symbol:STK25  
NCBI Official Synonym Symbols:SOK1; YSK1  
NCBI Protein Information:serine/threonine-protein kinase 25
UniProt Protein Name:Serine/threonine-protein kinase 25
UniProt Synonym Protein Names:Ste20-like kinase; Sterile 20/oxidant stress-response kinase 1; SOK-1; Ste20/oxidant stress response kinase 1
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:STK25  
UniProt Entry Name:STK25_HUMAN
Anti-STK25 Polyclonal Antibody (CAB9726)Western blot analysis of extracts of various cell lines, using STK25 antibody (CAB9726) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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