Cell Cycle Antibodies 1

Anti-STK10 Antibody (CAB14257)

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SKU:
CAB14257
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-STK10 Antibody
Antibody SKU:CAB14257
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 320-510 of human STK10 (NP_005981.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:K-562, U-87MG, HT-29, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 320-510 of human STK10 (NP_005981.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DGRD EGEE EDAV DAAS TLEN HTQN SSEV SPPS LNAD KPLE ESPS TPLA PSQS QDSV NEPC SQPS GDRS LQTT SPPV VAPG NENG LAVP VPLR KSRP VSMD ARIQ VAQE KQVA EQGG DLSP AANR SQKA SQSR PNSS ALET LGGE KLAN GSLE PPAQ AAPG PSKR DSDC SSLC TSES MDYG TNLS TDLS LNK
Gene ID:6793
Uniprot:O94804
Cellular Location:Cell membrane, Peripheral membrane protein
Calculated MW:112kDa
Observed MW:140kDa
Synonyms:STK10, LOK, PRO2729
Background:This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway.
UniProt Protein Function:LOK: a protein kinase of the STE20 family. Highly expressed in hematopoietic tissue. May cooperate with SLK to regulate Plk1 function in human cells.
UniProt Protein Details:

Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, STE; STE group; STE20 family; SLK subfamily

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: cytoplasm; plasma membrane

Molecular Function:identical protein binding; polo kinase kinase activity; protein binding; protein homodimerization activity; protein serine/threonine kinase activity

Biological Process: protein amino acid autophosphorylation; protein amino acid phosphorylation

NCBI Summary:This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. [provided by RefSeq, Jul 2008]
UniProt Code:O94804
NCBI GenInfo Identifier:12643358
NCBI Gene ID:6793
NCBI Accession:O94804.1
UniProt Secondary Accession:O94804,Q6NSK0, Q9UIW4, A6ND35, B2R8F5, B3KMY1,
UniProt Related Accession:O94804
Molecular Weight:112,135 Da
NCBI Full Name:Serine/threonine-protein kinase 10
NCBI Synonym Full Names:serine/threonine kinase 10
NCBI Official Symbol:STK10  
NCBI Official Synonym Symbols:LOK; PRO2729  
NCBI Protein Information:serine/threonine-protein kinase 10
UniProt Protein Name:Serine/threonine-protein kinase 10
UniProt Synonym Protein Names:Lymphocyte-oriented kinase
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:STK10  
UniProt Entry Name:STK10_HUMAN
Anti-STK10 Antibody (CAB14257)Western blot analysis of extracts of various cell lines, using STK10 antibody (CAB14257) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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