Cell Biology Antibodies 11
Anti-STIM1 Antibody (CAB7411)
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- SKU:
- CAB7411
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-STIM1 Antibody |
| Antibody SKU: | CAB7411 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 451-685 of human STIM1 (NP_003147.2). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, Jurkat, BxPC-3, HeLa, Mouse brain, Mouse heart, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 451-685 of human STIM1 (NP_003147.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VAAL NIDP SWMG STRP NPAH FIMT DDVD DMDE EIVS PLSM QSPS LQSS VRQR LTEP QHGL GSQR DLTH SDSE SSLH MSDR QRVA PKPP QMSR AADE ALNA MTSN GSHR LIEG VHPG SLVE KLPD SPAL AKKA LLAL NHGL DKAH SLME LSPS APPG GSPH LDSS RSHS PSSP DPDT PSPV GDSR ALQA SRNT RIPH LAGK KAVA EEDN GSIG EETD SSPG RKKF PLKI FKKP LKK |
| Gene ID: | 6786 |
| Uniprot: | Q13586 |
| Cellular Location: | Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Single-pass type I membrane protein, cytoskeleton |
| Calculated MW: | 62kDa/77kDa |
| Observed MW: | 100kDa |
| Synonyms: | STIM1, D11S4896E, GOK, IMD10, STRMK, TAM, TAM1 |
| Background: | This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. |
| UniProt Protein Function: | STIM1: Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. Forms homooligomers and heterooligomers with STIM2. Interacts with ORAI1. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with EFCAB4B/CRACR2A; the interaction is direct and takes place in absence of Ca(2+). Forms a complex with EFCAB4B/CRACR2A and ORAI1 at low concentration of Ca(2+), the complex dissociates at elevated Ca(2+) concentrations. Interacts with TMEM66/SARAF, promoting a slow inactivation of STIM1-dependent SOCE activity, possibly by facilitating the deoligomerization of STIM1. Ubiquitously expressed in various human primary cells and tumor cell lines. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Endoplasmic reticulum; Calcium-binding Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: microtubule; endoplasmic reticulum membrane; integral to plasma membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane Molecular Function:identical protein binding; microtubule plus-end binding; protein binding; calcium ion binding Biological Process: activation of store-operated calcium channel activity; calcium ion transport; regulation of calcium ion transport; detection of calcium ion; blood coagulation Disease: Myopathy, Tubular Aggregate, 1; Immunodeficiency 10 |
| NCBI Summary: | This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] |
| UniProt Code: | Q13586 |
| NCBI GenInfo Identifier: | 21070997 |
| NCBI Gene ID: | 6786 |
| NCBI Accession: | NP_003147.2 |
| UniProt Secondary Accession: | Q13586,Q8N382, E9PQJ4, |
| UniProt Related Accession: | Q13586 |
| Molecular Weight: | 62,133 Da |
| NCBI Full Name: | stromal interaction molecule 1 isoform 2 |
| NCBI Synonym Full Names: | stromal interaction molecule 1 |
| NCBI Official Symbol: | STIM1 |
| NCBI Official Synonym Symbols: | GOK; TAM; TAM1; IMD10; STRMK; D11S4896E |
| NCBI Protein Information: | stromal interaction molecule 1 |
| UniProt Protein Name: | Stromal interaction molecule 1 |
| UniProt Gene Name: | STIM1 |
| UniProt Entry Name: | STIM1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using STIM1 antibody (CAB7411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using STIM1 Rabbit pAb (CAB7411) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using STIM1 antibody (CAB7411) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using STIM1 antibody (CAB7411) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug STIM1 antibody (CAB7411). Western blot was performed from the immunoprecipitate using STIM1 antibody (CAB7411) at a dilition of 1:1000. |
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