Name: Anti-STIM1 Antibody (CAB19894)[KO Validated]
Brand: KO Validated Antibodies 2
SKU: CAB19894
Availability: OutOfStock

Antibody Name:	Anti-STIM1 Antibody [KO Validated]
Antibody SKU:	CAB19894
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human STIM1.

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant protein of human STIM1.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	6786
Uniprot:	Q13586
Cellular Location:	Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Single-pass type I membrane protein, cytoskeleton
Calculated MW:	62kDa/77kDa
Observed MW:	80KDa

Anti-STIM1 Antibody [KO Validated] (CAB19894)

Western blot analysis of extracts from normal (control) and STIM1 knockout (KO) HeLa cells, using STIM1 antibody (CAB19894) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Synonyms:

STIM1, D11S4896E, GOK, IMD10, STRMK, TAM, TAM1

Background:

This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants.

UniProt Protein Function:	STIM1: Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. Forms homooligomers and heterooligomers with STIM2. Interacts with ORAI1. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with EFCAB4B/CRACR2A; the interaction is direct and takes place in absence of Ca(2+). Forms a complex with EFCAB4B/CRACR2A and ORAI1 at low concentration of Ca(2+), the complex dissociates at elevated Ca(2+) concentrations. Interacts with TMEM66/SARAF, promoting a slow inactivation of STIM1-dependent SOCE activity, possibly by facilitating the deoligomerization of STIM1. Ubiquitously expressed in various human primary cells and tumor cell lines.
UniProt Protein Details:	Protein type:Membrane protein, integral; Endoplasmic reticulum; Calcium-binding Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: microtubule; endoplasmic reticulum membrane; integral to plasma membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane Molecular Function:identical protein binding; microtubule plus-end binding; protein binding; calcium ion binding Biological Process: activation of store-operated calcium channel activity; calcium ion transport; regulation of calcium ion transport; detection of calcium ion; blood coagulation Disease: Myopathy, Tubular Aggregate, 1; Immunodeficiency 10
NCBI Summary:	This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
UniProt Code:	Q13586
NCBI GenInfo Identifier:	21070997
NCBI Gene ID:	6786
NCBI Accession:	NP_003147.2
UniProt Secondary Accession:	Q13586,Q8N382, E9PQJ4,
UniProt Related Accession:	Q13586
Molecular Weight:	62,133 Da
NCBI Full Name:	stromal interaction molecule 1 isoform 2
NCBI Synonym Full Names:	stromal interaction molecule 1
NCBI Official Symbol:	STIM1
NCBI Official Synonym Symbols:	GOK; TAM; TAM1; IMD10; STRMK; D11S4896E
NCBI Protein Information:	stromal interaction molecule 1
UniProt Protein Name:	Stromal interaction molecule 1
UniProt Gene Name:	STIM1
UniProt Entry Name:	STIM1_HUMAN