Epigenetics & Nuclear Signaling Antibodies 4
Anti-STAT4 Antibody (CAB6991)
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- SKU:
- CAB6991
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-STAT4 Antibody |
| Antibody SKU: | CAB6991 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 569-748 of human STAT4 (NP_003142.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NCI-H460 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 569-748 of human STAT4 (NP_003142.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | WIDG YVMG FVSK EKER LLLK DKMP GTFL LRFS ESHL GGIT FTWV DHSE SGEV RFHS VEPY NKGR LSAL PFAD ILRD YKVI MAEN IPEN PLKY LYPD IPKD KAFG KHYS SQPC EVSR PTER GDKG YVPS VFIP ISTI RSDS TEPH SPSD LLPM SPSV YAVL RENL SPTT IETA MKSP YSAE |
| Gene ID: | 6775 |
| Uniprot: | Q14765 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 85kDa |
| Observed MW: | 76kDa |
| Synonyms: | STAT4, SLEB11 |
| Background: | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. |
| UniProt Protein Function: | STAT4: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 2q32.2-q32.3 Cellular Component: nucleoplasm; cytoplasm Molecular Function:protein binding; signal transducer activity; sequence-specific DNA binding; transcription factor activity Biological Process: cell proliferation; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter; JAK-STAT cascade; protein amino acid phosphorylation Disease: Systemic Lupus Erythematosus, Susceptibility To, 11 |
| NCBI Summary: | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q14765 |
| NCBI GenInfo Identifier: | 6226158 |
| NCBI Gene ID: | 6775 |
| NCBI Accession: | Q14765.1 |
| UniProt Secondary Accession: | Q14765,Q96NZ6, |
| UniProt Related Accession: | Q14765 |
| Molecular Weight: | 85,941 Da |
| NCBI Full Name: | Signal transducer and activator of transcription 4 |
| NCBI Synonym Full Names: | signal transducer and activator of transcription 4 |
| NCBI Official Symbol: | STAT4 |
| NCBI Official Synonym Symbols: | SLEB11 |
| NCBI Protein Information: | signal transducer and activator of transcription 4 |
| UniProt Protein Name: | Signal transducer and activator of transcription 4 |
| Protein Family: | Protein slender lobes |
| UniProt Gene Name: | STAT4 |
| UniProt Entry Name: | STAT4_HUMAN |
 | Western blot analysis of extracts of NCI-H460 cells, using STAT4 antibody (CAB6991) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat spleen using STAT4 antibody (CAB6991) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human rectum using STAT4 antibody (CAB6991) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using STAT4 antibody (CAB6991) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of MCF7 cells using STAT4 antibody (CAB6991). Blue: DAPI for nuclear staining. |
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