Antibody Name:	Anti-STAR Antibody
Antibody SKU:	CAB16432
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human STAR

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	K-562, Mouse ovary, Rat testis

Immunogen:	Recombinant protein of human STAR
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	6770
Uniprot:	P49675
Cellular Location:	Mitochondrion
Calculated MW:	31kDa
Observed MW:	28kDa

Synonyms:

STAR, STARD1, STAR

Background:

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.

UniProt Protein Function:	STAR: Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone. May interact with TSPO. Expressed in gonads, adrenal cortex and kidney.
UniProt Protein Details:	Protein type:Mitochondrial; Lipid-binding; Carrier Chromosomal Location of Human Ortholog: 8p11.2 Cellular Component: mitochondrial crista; neuron projection; cell soma; mitochondrial intermembrane space; cytosol Molecular Function:cholesterol transporter activity; cholesterol binding Biological Process: response to nicotine; steroid metabolic process; diterpenoid metabolic process; dibenzo-p-dioxin metabolic process; regulation of steroid biosynthetic process; estrogen biosynthetic process; biphenyl metabolic process; circadian sleep/wake cycle, REM sleep; negative regulation of neuron apoptosis; response to nutrient; response to corticosterone stimulus; response to drug; cholesterol metabolic process; bile acid biosynthetic process; positive regulation of neurogenesis; insecticide metabolic process; progesterone biosynthetic process; fractalkine metabolic process; male gonad development; phthalate metabolic process; intracellular cholesterol transport; response to herbicide; glucocorticoid metabolic process; response to ethanol; response to hydrogen peroxide; cellular response to insulin stimulus; response to estrogen stimulus; response to lead ion; C21-steroid hormone biosynthetic process; brain development; response to activity; response to ionizing radiation; steroid biosynthetic process; regulation of neuronal synaptic plasticity; phenol metabolic process Disease: Lipoid Congenital Adrenal Hyperplasia
NCBI Summary:	The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq, Jul 2008]
UniProt Code:	P49675
NCBI GenInfo Identifier:	71152974
NCBI Gene ID:	6770
NCBI Accession:	P49675.2
UniProt Secondary Accession:	P49675,Q16396,
UniProt Related Accession:	P49675
Molecular Weight:	285
NCBI Full Name:	Steroidogenic acute regulatory protein, mitochondrial
NCBI Synonym Full Names:	steroidogenic acute regulatory protein
NCBI Official Symbol:	STAR
NCBI Official Synonym Symbols:	STARD1
NCBI Protein Information:	steroidogenic acute regulatory protein, mitochondrial; cholesterol trafficker; START domain containing 1; steroid acute regulatory protein; START domain-containing protein 1; mitochondrial steroid acute regulatory protein; StAR-related lipid transfer (START) domain containing 1
UniProt Protein Name:	Steroidogenic acute regulatory protein, mitochondrial
UniProt Synonym Protein Names:	START domain-containing protein 1; StARD1
Protein Family:	Starch synthase
UniProt Gene Name:	STAR
UniProt Entry Name:	STAR_HUMAN

	Western blot analysis of extracts of various cell lines, using STAR antibody (CAB16432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of L929 cells using STAR Polyclonal Antibody (CAB16432) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using STAR Polyclonal Antibody (CAB16432) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Confocal immunofluorescence analysis of mouse adrenal gland using STAR Polyclonal Antibody (CAB16432) at dilution of 1:100. Blue: DAPI for nuclear staining.