Name: Anti-STAP2 Antibody (CAB16131)
Brand: Cell Biology Antibodies 6
SKU: CAB16131
Availability: OutOfStock

Antibody Name:	Anti-STAP2 Antibody
Antibody SKU:	CAB16131
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 130-220 of human STAP2 (NP_001013863).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	Mouse heart, HT-29

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 130-220 of human STAP2 (NP_001013863).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GHLY MMSE VLAK EEAR RALE TPSC FLKV SRLE AQLL LERY PECG NLLL RPSG DGAD GVSV TTRQ MHNG THVV RHYK VKRE GPKY VIDV EQP
Gene ID:	55620
Uniprot:	Q9UGK3
Cellular Location:	Cytoplasm
Calculated MW:	44kDa/49kDa
Observed MW:	44kDa

Synonyms:	STAP2, BKS
Background:	This gene encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	STAP2: a widely expressed proimflammatory adaptor protein that regulates immune and inflammatory responses. Binds and modulates a variety of molecules including Brk, CSFR, FCER1, and BCR-ABL. The interaction between STAP-2 and BCR-ABL appears to provide a growth advantage to BCR-ABL+ chronic myeloid leukemia cells. Suppression of STAP-2 expression in K562 CML cells suppressed tumor formation in mice. Enables intestinal proinflammatory responses in vivo: promotes macrophage recruitment in dextran sodium sulfate induced colitis, and promotes the ability of CXCL12-induced migration in macrophages. STAP-2 knockdown in T47D breast cancer cells induced a significant decrease of proliferation. Contains a pleckstrin homology, Src homology 2-like, and as well as a STAT3-binding domains. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: cytosol; plasma membrane Molecular Function:protein binding Biological Process: positive regulation of tyrosine phosphorylation of Stat3 protein
NCBI Summary:	This gene encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UGK3
NCBI GenInfo Identifier:	229462752
NCBI Gene ID:	55620
NCBI Accession:	Q9UGK3.2
UniProt Secondary Accession:	Q9UGK3,Q9NXI2, A6NKK3,
UniProt Related Accession:	Q9UGK3
Molecular Weight:	49,649 Da
NCBI Full Name:	Signal-transducing adaptor protein 2
NCBI Synonym Full Names:	signal transducing adaptor family member 2
NCBI Official Symbol:	STAP2
NCBI Official Synonym Symbols:	BKS
NCBI Protein Information:	signal-transducing adaptor protein 2
UniProt Protein Name:	Signal-transducing adaptor protein 2
UniProt Synonym Protein Names:	Breast tumor kinase substrate; BRK substrate
Protein Family:	Signal-transducing adaptor protein
UniProt Gene Name:	STAP2
UniProt Entry Name:	STAP2_HUMAN

	Western blot analysis of extracts of Mouse heart, using STAP2 antibody (CAB16131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts of HT-29 cells, using STAP2 antibody (CAB16131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 240s.