Antibody Name:	Anti-STAM2 Antibody
Antibody SKU:	CAB7058
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 376-525 of human STAM2 (NP_005834.4).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HepG2, OVCAR3, PC-3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 376-525 of human STAM2 (NP_005834.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KLHP PAHY PPAS SGVP MQTY PVQS HGGN YMGQ SIHQ VTVA QSYS LGPD QIGP LRSL PPNV NSSV TAQP AQTS YLST GQDT VSNP TYMN QNSN LQSA TGTT AYTQ QMGM SVDM SSYQ NTTS NLPQ LAGF PVTV PAHP VAQQ HTNY HQQP LL
Gene ID:	10254
Uniprot:	O75886
Cellular Location:	Cytoplasm, Cytoplasmic side, Early endosome membrane, Peripheral membrane protein
Calculated MW:	38kDa/58kDa
Observed MW:	70kDa

Synonyms:

STAM2, Hbp, STAM2A, STAM2B

Background:

The protein encoded by this gene is closely related to STAM, an adaptor protein involved in the downstream signaling of cytokine receptors, both of which contain a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). Similar to STAM, this protein acts downstream of JAK kinases, and is phosphorylated in response to cytokine stimulation. This protein and STAM thus are thought to exhibit compensatory effects on the signaling pathway downstream of JAK kinases upon cytokine stimulation.

UniProt Protein Function:	STAM2: an adaptor protein involved in the downstream signaling of cytokine receptors. Contain a SH3 domain and immunoreceptor tyrosine-based activation motif (ITAM). Acts downstream of JAK kinases, and is phosphorylated in response to cytokine stimulation. May modulate the signaling pathway downstream of JAK kinases upon cytokine stimulation.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2q23.3 Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; nucleoplasm Molecular Function:protein binding Biological Process: autophagy; endosome transport; negative regulation of epidermal growth factor receptor signaling pathway
NCBI Summary:	The protein encoded by this gene is closely related to STAM, an adaptor protein involved in the downstream signaling of cytokine receptors, both of which contain a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). Similar to STAM, this protein acts downstream of JAK kinases, and is phosphorylated in response to cytokine stimulation. This protein and STAM thus are thought to exhibit compensatory effects on the signaling pathway downstream of JAK kinases upon cytokine stimulation. [provided by RefSeq, Jul 2008]
UniProt Code:	O75886
NCBI GenInfo Identifier:	71153548
NCBI Gene ID:	10254
NCBI Accession:	O75886.1
UniProt Secondary Accession:	O75886,Q7LDQ0, Q9UF58, A8K8A0, D3DPA1,
UniProt Related Accession:	O75886
Molecular Weight:	38,370 Da
NCBI Full Name:	Signal transducing adapter molecule 2
NCBI Synonym Full Names:	signal transducing adaptor molecule 2
NCBI Official Symbol:	STAM2
NCBI Official Synonym Symbols:	Hbp; STAM2A; STAM2B
NCBI Protein Information:	signal transducing adapter molecule 2
UniProt Protein Name:	Signal transducing adapter molecule 2
UniProt Synonym Protein Names:	Hrs-binding protein
Protein Family:	Signal transducing adapter molecule
UniProt Gene Name:	STAM2
UniProt Entry Name:	STAM2_HUMAN

	Western blot analysis of extracts of various cell lines, using STAM2 antibody (CAB7058) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of C6 cells using STAM2 antibody (CAB7058) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using STAM2 antibody (CAB7058) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using STAM2 antibody (CAB7058) at dilution of 1:100. Blue: DAPI for nuclear staining.