Name: Anti-STAM Antibody (CAB4198)
Brand: Cell Biology Antibodies 9
SKU: CAB4198
Availability: OutOfStock

Antibody Name:	Anti-STAM Antibody
Antibody SKU:	CAB4198
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-210 of human STAM (NP_003464.1).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:1000 IHC 1:20 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, Jurkat, HeLa, HepG2, Mouse pancreas, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-210 of human STAM (NP_003464.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KNDP QLSL ISAM IKNL KEQG VTFP AIGS QAAE QAKA SPAL VAKD PGTV ANKK EEED LAKA IELS LKEQ RQQS TTLS TLYP STSS LLTN HQH
Gene ID:	8027
Uniprot:	Q92783
Cellular Location:	Cytoplasm, Cytoplasmic side, Early endosome membrane, Peripheral membrane protein
Calculated MW:	44kDa/59kDa
Observed MW:	70kDa

Synonyms:

STAM, STAM-1, STAM1

Background:

This gene encodes a member of the signal-transducing adaptor molecule family. These proteins mediate downstream signaling of cytokine receptors and also play a role in ER to Golgi trafficking by interacting with the coat protein II complex. The encoded protein also associates with hepatocyte growth factor-regulated substrate to form the endosomal sorting complex required for transport-0 (ESCRT-0), which sorts ubiquitinated membrane proteins to the ESCRT-1 complex for lysosomal degradation. Alternatively spliced transcript variants have been observed for this gene.

UniProt Protein Function:	STAM1: Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. Belongs to the STAM family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 10p14-p13 Cellular Component: early endosome membrane; cytosol Molecular Function:protein binding; SH3/SH2 adaptor activity Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; intracellular protein transport; positive regulation of signal transduction; signal transduction; endosome transport
NCBI Summary:	This gene encodes a member of the signal-transducing adaptor molecule family. These proteins mediate downstream signaling of cytokine receptors and also play a role in ER to Golgi trafficking by interacting with the coat protein II complex. The encoded protein also associates with hepatocyte growth factor-regulated substrate to form the endosomal sorting complex required for transport-0 (ESCRT-0), which sorts ubiquitinated membrane proteins to the ESCRT-1 complex for lysosomal degradation. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Feb 2011]
UniProt Code:	Q92783
NCBI GenInfo Identifier:	71153545
NCBI Gene ID:	8027
NCBI Accession:	Q92783.3
UniProt Related Accession:	Q92783
Molecular Weight:
NCBI Full Name:	Signal transducing adapter molecule 1
NCBI Synonym Full Names:	signal transducing adaptor molecule
NCBI Official Symbol:	STAM
NCBI Official Synonym Symbols:	STAM1; STAM-1
NCBI Protein Information:	signal transducing adapter molecule 1
UniProt Protein Name:	Signal transducing adapter molecule 1
Protein Family:	STAM-binding protein
UniProt Gene Name:	STAM
UniProt Entry Name:	STAM1_HUMAN

Western blot analysis of extracts of various cell lines, using STAM antibody (CAB4198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

Antibodies

STAM Antibody (PACO12501)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)