Cell Cycle Antibodies 1

Anti-STAG1 Antibody (CAB17315)

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SKU:
CAB17315
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-STAG1 Antibody
Antibody SKU:CAB17315
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 930-1140 of human STAG1 (NP_005853.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa, Jurkat, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 930-1140 of human STAG1 (NP_005853.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LFNE LVQE QGPN LDRT SAHV SGIK ELAR RFAL TFGL DQIK TREA VATL HKDG IEFA FKYQ NQKG QEYP PPNL AFLE VLSE FSSK LLRQ DKKT VHSY LEKF LTEQ MMER REDV WLPL ISYR NSLV TGGE DDRM SVNS GSSS SKTS SVRN KKGR PPLH KKRV EDES LDNT WLNR TDTM IQTP GPLP APQL TSTV LREN SRPM GDQI QEPE SEH
Gene ID:10274
Uniprot:Q8WVM7
Cellular Location:
Calculated MW:144kDa
Observed MW:144kDa
Synonyms:STAG1, SA1, SCC3A, MRD47
Background:This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.
UniProt Protein Function:STAG1: Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Belongs to the SCC3 family.
UniProt Protein Details:

Protein type:Apoptosis

Chromosomal Location of Human Ortholog: 3q22.3

Cellular Component: nucleoplasm; chromosome; nucleus; cytosol; chromatin; chromosome, pericentric region

Molecular Function:protein binding; chromatin binding

Biological Process: mitosis; cell division; mitotic cell cycle; chromosome segregation

NCBI Summary:This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq, Jul 2008]
UniProt Code:Q8WVM7
NCBI GenInfo Identifier:209572720
NCBI Gene ID:10274
NCBI Accession:Q8WVM7.3
UniProt Secondary Accession:Q8WVM7,O00539, Q6P275,
UniProt Related Accession:Q8WVM7
Molecular Weight:1258
NCBI Full Name:Cohesin subunit SA-1
NCBI Synonym Full Names:stromal antigen 1
NCBI Official Symbol:STAG1  
NCBI Official Synonym Symbols:SA1; SCC3A  
NCBI Protein Information:cohesin subunit SA-1; SCC3 homolog 1; nuclear protein stromal antigen 1
UniProt Protein Name:Cohesin subunit SA-1
UniProt Synonym Protein Names:SCC3 homolog 1; Stromal antigen 1
UniProt Gene Name:STAG1  
UniProt Entry Name:STAG1_HUMAN
Anti-STAG1 Antibody (CAB17315)Western blot analysis of extracts of various cell lines, using STAG1 antibody (CAB17315) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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