Name: Anti-ST3GAL5 Antibody (CAB7947)
Brand: Cell Biology Antibodies 11
SKU: CAB7947
Availability: OutOfStock

Antibody Name:	Anti-ST3GAL5 Antibody
Antibody SKU:	CAB7947
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-200 of human ST3GAL5 (NP_003887.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, U-251MG, SH-SY5Y, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-200 of human ST3GAL5 (NP_003887.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LYIL KLNY TTEE CDMK KMHY VDPD HVKR AQKY AQQV LQKE CRPK FAKT SMAL LFEH RYSV DLLP FVQK APKD SEAE SKYD PPFG FRKF SSKV QTLL ELLP EHDL PEHL KAKT CRRC VVIG S
Gene ID:	8869
Uniprot:	Q9UNP4
Cellular Location:	Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:	45kDa/47kDa
Observed MW:	48kDa

Synonyms:

ST3GAL5, SATI, SIAT9, SIATGM3S, SPDRS, ST3GalV

Background:

Ganglioside GM3 is known to participate in the induction of cell differentiation, modulation of cell proliferation, maintenance of fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. The protein encoded by this gene is a type II membrane protein which catalyzes the formation of GM3 using lactosylceramide as the substrate. The encoded protein is a member of glycosyltransferase family 29 and may be localized to the Golgi apparatus. Mutation in this gene has been associated with Amish infantile epilepsy syndrome. Transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ST3GAL5: Catalyzes the formation of ganglioside GM3 (alpha-N- acetylneuraminyl-2,3-beta-D-galactosyl-1, 4-beta-D- glucosylceramide). Defects in ST3GAL5 are the cause of Amish infantile epilepsy syndrome (AIES). AIES is an autosomal recessive, infantile-onset symptomatic epilepsy associated with developmental stagnation and blindness. Belongs to the glycosyltransferase 29 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - ganglio series; EC 2.4.99.9; Transferase Chromosomal Location of Human Ortholog: 2p11.2 Cellular Component: Golgi membrane; integral to plasma membrane; integral to membrane; integral to Golgi membrane Molecular Function:lactosylceramide alpha-2,3-sialyltransferase activity; neolactotetraosylceramide alpha-2,3-sialyltransferase activity; sialyltransferase activity Biological Process: cellular protein metabolic process; glycosphingolipid biosynthetic process; dolichol-linked oligosaccharide biosynthetic process; carbohydrate metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; ganglioside biosynthetic process Disease: Amish Infantile Epilepsy Syndrome
NCBI Summary:	Ganglioside GM3 is known to participate in the induction of cell differentiation, modulation of cell proliferation, maintenance of fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. The protein encoded by this gene is a type II membrane protein which catalyzes the formation of GM3 using lactosylceramide as the substrate. The encoded protein is a member of glycosyltransferase family 29 and may be localized to the Golgi apparatus. Mutation in this gene has been associated with Amish infantile epilepsy syndrome. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UNP4
NCBI GenInfo Identifier:	109633044
NCBI Gene ID:	8869
NCBI Accession:	NP_003887.3
UniProt Secondary Accession:	Q9UNP4,O94902, Q53QU1, Q6NZX4, Q6YFL1, B3KM82, D6W5L9
UniProt Related Accession:	Q9UNP4
Molecular Weight:	45,585 Da
NCBI Full Name:	lactosylceramide alpha-2,3-sialyltransferase isoform 1
NCBI Synonym Full Names:	ST3 beta-galactoside alpha-2,3-sialyltransferase 5
NCBI Official Symbol:	ST3GAL5
NCBI Official Synonym Symbols:	SATI; SIAT9; ST3GalV; SIATGM3S
NCBI Protein Information:	lactosylceramide alpha-2,3-sialyltransferase
UniProt Protein Name:	Lactosylceramide alpha-2,3-sialyltransferase
UniProt Synonym Protein Names:	CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase; Ganglioside GM3 synthase; ST3Gal V; ST3GalV; Sialyltransferase 9
Protein Family:	Lactosylceramide alpha-2,3-sialyltransferase
UniProt Gene Name:	ST3GAL5
UniProt Entry Name:	SIAT9_HUMAN

Western blot analysis of extracts of various cell lines, using ST3GAL5 antibody (CAB7947) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

Antibodies

ST3GAL5 Antibody (PACO12488)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)