Antibody Name:	Anti-ST3GAL4 Antibody
Antibody SKU:	CAB6309
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 27-267 of human ST3GAL4 (NP_006269.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Rat
Positive Samples:	HT-29, Rat testis, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 27-267 of human ST3GAL4 (NP_006269.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	REDS FYFP IPEK KEPC LQGE AESK ASKL FGNY SRDQ PIFL RLED YFWV KTPS AYEL PYGT KGSE DLLL RVLA ITSS SIPK NIQS LRCR RCVV VGNG HRLR NSSL GDAI NKYD VVIR LNNA PVAG YEGD VGSK TTMR LFYP ESAH FDPK VENN PDTL LVLV AFKA MDFH WIET ILSD KKRV RKGF WKQP PLIW DVNP KQIR ILNP FFME IAAD KLLS LPMQ QPRK IKQK PTTG LLAI TLAL H
Gene ID:	6484
Uniprot:	Q11206
Cellular Location:	Golgi apparatus, Golgi stack membrane, Secreted, Single-pass type II membrane protein
Calculated MW:	36kDa/37kDa/38kDa
Observed MW:	40kDa

Synonyms:

ST3GAL4, CGS23, NANTA3, SAT3, SIAT4, SIAT4C, ST-4, ST3GalA.2, ST3GalIV, STZ, gal-NAc6S

Background:

This gene encodes a member of the glycosyltransferase 29 family, a group of enzymes involved in protein glycosylation. The encoded protein is targeted to Golgi membranes but may be proteolytically processed and secreted. The gene product may also be involved in the increased expression of sialyl Lewis X antigen seen in inflammatory responses. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ST3GAL4: It may catalyze the formation of the NeuAc-alpha-2,3- Gal-beta-1,3-GalNAc- or NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. It may be involved in the biosynthesis of the sialyl Lewis X determinant. Also acts on the corresponding 1,3- galactosyl derivative. Belongs to the glycosyltransferase 29 family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; EC 2.4.99.-; Glycan Metabolism - keratan sulfate biosynthesis; Transferase; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series Chromosomal Location of Human Ortholog: 11q24.2 Cellular Component: Golgi membrane; membrane Molecular Function:beta-galactoside alpha-2,3-sialyltransferase activity; monosialoganglioside sialyltransferase activity Biological Process: cognition; ganglioside biosynthetic process; keratan sulfate biosynthetic process; O-glycan processing; oligosaccharide metabolic process; protein amino acid N-linked glycosylation via asparagine
NCBI Summary:	This gene encodes a member of the glycosyltransferase 29 family, a group of enzymes involved in protein glycosylation. The encoded protein is targeted to Golgi membranes but may be proteolytically processed and secreted. The gene product may also be involved in the increased expression of sialyl Lewis X antigen seen in inflammatory responses. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	Q11206
NCBI GenInfo Identifier:	1705565
NCBI Gene ID:	6484
NCBI Accession:	Q11206.1
UniProt Secondary Accession:	Q11206,O60497, Q8N6A6, Q8NFG7, Q96QQ9, A8K6B2,
UniProt Related Accession:	Q11206
Molecular Weight:	36,157 Da
NCBI Full Name:	CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4
NCBI Synonym Full Names:	ST3 beta-galactoside alpha-2,3-sialyltransferase 4
NCBI Official Symbol:	ST3GAL4
NCBI Official Synonym Symbols:	STZ; SAT3; CGS23; SIAT4; NANTA3; SIAT4C; ST3GalIV
NCBI Protein Information:	CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4
UniProt Protein Name:	CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4
UniProt Synonym Protein Names:	Alpha 2,3-sialyltransferase IV; Gal-NAc6S; Gal-beta-1,4-GalNAc-alpha-2,3-sialyltransferase; SAT-3; ST-4; ST3Gal IV; ST3GalIV; ST3GalA.2; STZ; Sialyltransferase 4C; SIAT4-C
Protein Family:	CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase
UniProt Gene Name:	ST3GAL4
UniProt Entry Name:	SIA4C_HUMAN

	Western blot analysis of extracts of various cell lines, using ST3GAL4 antibody (CAB6309) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
	Immunofluorescence analysis of HeLa cells using ST3GAL4 antibody (CAB6309). Blue: DAPI for nuclear staining.