Name: Anti-ST3GAL2 Antibody (CAB13733)
Brand: Cell Biology Antibodies 4
SKU: CAB13733
Availability: OutOfStock

Antibody Name:	Anti-ST3GAL2 Antibody
Antibody SKU:	CAB13733
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-210 of human ST3GAL2 (NP_008858.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, Mouse heart, Mouse kidney, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-210 of human ST3GAL2 (NP_008858.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SMAT LPYL DSGA LDGT HRVK LVPG YAGL QRLS KERL SGKS CACR RCMG DAGA SDWF DSHF DGNI SPVW TREN MDLP PDVQ RWWM MLQP QFKS HNTN EVLE KLFQ IVPG ENPY RFRD PHQC RRCA VVGN SGNL RGSG YGQD VDGH NFIM RMNQ APTV GFEQ DVGS RTTH HFMY PESA KNLP A
Gene ID:	6483
Uniprot:	Q16842
Cellular Location:	Golgi apparatus, Golgi stack membrane, Secreted, Single-pass type II membrane protein
Calculated MW:	40kDa
Observed MW:	40-45kDa

Synonyms:

ST3GAL2, Gal-NAc6S, SIAT4B, ST3GALII, ST3GalA.2

Background:

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4A.

UniProt Protein Function:	ST3GAL2: It may be responsible for the synthesis of the sequence NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- found in terminal carbohydrate groups of certain glycoproteins, oligosaccharides and glycolipids. SIAT4A and SIAT4B sialylate the same acceptor substrates but exhibit different Km values. Belongs to the glycosyltransferase 29 family.
UniProt Protein Details:	Protein type:Membrane protein, integral; EC 2.4.99.4; Glycan Metabolism - O-glycan biosynthesis; Glycan Metabolism - glycosphingolipid biosynthesis - globo series; Glycan Metabolism - keratan sulfate biosynthesis; Transferase; Glycan Metabolism - glycosphingolipid biosynthesis - ganglio series Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: Golgi membrane Molecular Function:beta-galactoside alpha-2,3-sialyltransferase activity Biological Process: amino sugar metabolic process; ganglioside biosynthetic process; keratan sulfate biosynthetic process; O-glycan processing; oligosaccharide metabolic process; protein amino acid N-linked glycosylation via asparagine; protein modification process
NCBI Summary:	The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4A. [provided by RefSeq, Jul 2008]
UniProt Code:	Q16842
NCBI GenInfo Identifier:	21759433
NCBI Gene ID:	6483
NCBI Accession:	Q16842.1
UniProt Secondary Accession:	Q16842,O00654,
UniProt Related Accession:	Q16842
Molecular Weight:	40,173 Da
NCBI Full Name:	CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2
NCBI Synonym Full Names:	ST3 beta-galactoside alpha-2,3-sialyltransferase 2
NCBI Official Symbol:	ST3GAL2
NCBI Official Synonym Symbols:	SIAT4B; ST3GALII; Gal-NAc6S; ST3GalA.2
NCBI Protein Information:	CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2
UniProt Protein Name:	CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2
UniProt Synonym Protein Names:	Gal-NAc6S; Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase; ST3Gal II; ST3GalII; ST3GalA.2; Sialyltransferase 4B; SIAT4-B
Protein Family:	CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase
UniProt Gene Name:	ST3GAL2
UniProt Entry Name:	SIA4B_HUMAN

Western blot analysis of extracts of various cell lines, using ST3GAL2 antibody (CAB13733) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

ST3GAL2 Antibody (PACO12486)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)