Epigenetics & Nuclear Signaling Antibodies 4
Anti-SSX5 Antibody (CAB7732)
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- SKU:
- CAB7732
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-SSX5 Antibody |
| Antibody SKU: | CAB7732 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-229 of human SSX5 (NP_066295.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-229 of human SSX5 (NP_066295.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MNGD DAFV RRPR VGSQ IPEK MQKH PWRQ VCDR GIHL VNLS PFWK VGRE PASS IKAL LCGR GEAR AFDD IAKY FSEK EWEK MKAS EKII YVYM KRKY EAMT KLGF KATL PPFM RNKR VADF QGND FDND PNRG NQVE HPQM TFGR LQGI FPKI TPEK PAEE GNDS KGVP EASG PQNN GKQL RPSG KLNT SEKV NKTS GPKR GKHA WTHR VRER KQLV IYEE ISDP QEDD E |
| Gene ID: | 6758 |
| Uniprot: | O60225 |
| Cellular Location: | |
| Calculated MW: | 21kDa/26kDa |
| Observed MW: | 22kDa |
| Synonyms: | SSX5 |
| Background: | The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneous humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. While some of the related SSX genes are involved in t(X;18)(p11.2;q11.2) translocations that are characteristically found in all synovial sarcomas, this gene does not appear to be involved in such translocations. Two transcript variants encoding distinct isoforms have been identified for this gene. |
| UniProt Protein Function: | Ssxb8: Could act as a modulator of transcription. Belongs to the SSX family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cancer Testis Antigen (CTA) Chromosomal Location of Human Ortholog: Xp11.23 Cellular Component: nucleus Molecular Function:protein binding; transcription corepressor activity |
| NCBI Summary: | The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneous humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. While some of the related SSX genes are involved in t(X;18)(p11.2;q11.2) translocations that are characteristically found in all synovial sarcomas, this gene does not appear to be involved in such translocations. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2013] |
| UniProt Code: | O60225 |
| NCBI GenInfo Identifier: | 115502458 |
| NCBI Gene ID: | 6758 |
| NCBI Accession: | O60225.3 |
| UniProt Secondary Accession: | O60225,Q5JQ59, Q5JQ60, Q96AW3, |
| UniProt Related Accession: | O60225 |
| Molecular Weight: | 26,284 Da |
| NCBI Full Name: | Protein SSX5 |
| NCBI Synonym Full Names: | SSX family member 5 |
| NCBI Official Symbol: | SSX5 |
| NCBI Protein Information: | protein SSX5 |
| UniProt Protein Name: | Protein SSX5 |
| Protein Family: | Protein |
| UniProt Gene Name: | SSX5 |
| UniProt Entry Name: | SSX5_HUMAN |
 | Western blot analysis of extracts of Rat liver, using SSX5 Rabbit pAb (CAB7732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse liver using SSX5 antibody (CAB7732) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using SSX5 antibody (CAB7732). Blue: DAPI for nuclear staining. |
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