Name: Anti-SSX4 Antibody (CAB7929)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB7929
Availability: OutOfStock

Antibody Name:	Anti-SSX4 Antibody
Antibody SKU:	CAB7929
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 70-188 of human SSX4 (NP_005627.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	Mouse kidney, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 70-188 of human SSX4 (NP_005627.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FMRS KRAA DFHG NDFG NDRN HRNQ VERP QMTF GSLQ RIFP KIMP KKPA EEEN GLKE VPEA SGPQ NDGK QLCP PGNP STLE KINK TSGP KRGK HAWT HRLR ERKQ LVVY EEIS DPEE DDE
Gene ID:	6759
Uniprot:	O60224
Cellular Location:
Calculated MW:	17kDa/21kDa
Observed MW:	27kDa

Synonyms:

SSX4, CT5.4

Background:

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. Chromosome Xp11 contains a segmental duplication resulting in two identical copies of synovial sarcoma, X breakpoint 4, SSX4 and SSX4B, in tail-to-tail orientation. This gene, SSX4, represents the more telomeric copy. Two transcript variants encoding distinct isoforms have been identified for this gene.

UniProt Protein Function:	SSX4: Could act as a modulator of transcription. Belongs to the SSX family.
UniProt Protein Details:	Protein type:Cancer Testis Antigen (CTA) Chromosomal Location of Human Ortholog: Xp11.23 Cellular Component: nucleus Molecular Function:transcription corepressor activity
NCBI Summary:	The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. Chromosome Xp11 contains a segmental duplication resulting in two identical copies of synovial sarcoma, X breakpoint 4, SSX4 and SSX4B, in tail-to-tail orientation. This gene, SSX4B, represents the more centromeric copy. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O60224
NCBI GenInfo Identifier:	6094362
NCBI Gene ID:	548313
NCBI Accession:	O60224.1
UniProt Secondary Accession:	O60224,Q3SYD4, Q5JQZ0, Q9UJU9, A8MYD4, B2RPE3,
UniProt Related Accession:	O60224
Molecular Weight:	17,918 Da
NCBI Full Name:	Protein SSX4
NCBI Synonym Full Names:	SSX family member 4B
NCBI Official Symbol:	SSX4B
NCBI Official Synonym Symbols:	CT5.4
NCBI Protein Information:	protein SSX4
UniProt Protein Name:	Protein SSX4
UniProt Synonym Protein Names:	Cancer/testis antigen 5.4; CT5.4
Protein Family:	Protein
UniProt Gene Name:	SSX4
UniProt Entry Name:	SSX4_HUMAN

Western blot analysis of extracts of various cell lines, using SSX4 antibody (CAB7929) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

SSX4 Antibody (PACO12484)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)