Anti-SSRP1 Antibody (CAB6413)

Antibody Name:	Anti-SSRP1 Antibody
Antibody SKU:	CAB6413
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SSRP1 (NP_003137.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	HT-29, SW620, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SSRP1 (NP_003137.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAET LEFN DVYQ EVKG SMND GRLR LSRQ GIIF KNSK TGKV DNIQ AGEL TEGI WRRV ALGH GLKL LTKN GHVY KYDG FRES EFEK LSDF FKTH YRLE LMEK DLCV KGWN WGTV KFGG QLLS FDIG DQPV FEIP LSNV SQCT TGKN EVTL EFHQ NDDA EVSL MEVR FYVP PTQE DGVD PVEA FAQN VLSK ADVI QATG DAIC IFRE LQCL TPRG RYDI RIYP TFLH LHGK TFDY KIPY TTVL RLFL LPHK DQRQ MFFV ISLD PPIK QGQT RYHF LILL FSKD EDIS LTLN MNEE EVEK RFEG
Gene ID:	6749
Uniprot:	Q08945
Cellular Location:	Chromosome, Nucleus, nucleolus
Calculated MW:	81kDa
Observed MW:	98kDa

Synonyms:

SSRP1, FACT, FACT80, T160

Background:

The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known.

UniProt Protein Function:	SSRP1: a subunit of the chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63.
UniProt Protein Details:	Protein type:RNA-binding; Nucleolus; Transcription initiation complex; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 11q12 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter
NCBI Summary:	The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known. [provided by RefSeq, Jul 2008]
UniProt Code:	Q08945
NCBI GenInfo Identifier:	730840
NCBI Gene ID:	6749
NCBI Accession:	Q08945.1
UniProt Secondary Accession:	Q08945,Q5BJG8,
UniProt Related Accession:	Q08945
Molecular Weight:	81,075 Da
NCBI Full Name:	FACT complex subunit SSRP1
NCBI Synonym Full Names:	structure specific recognition protein 1
NCBI Official Symbol:	SSRP1
NCBI Official Synonym Symbols:	FACT; T160; FACT80
NCBI Protein Information:	FACT complex subunit SSRP1
UniProt Protein Name:	FACT complex subunit SSRP1
UniProt Synonym Protein Names:	Chromatin-specific transcription elongation factor 80 kDa subunit; Facilitates chromatin transcription complex 80 kDa subunit; FACT 80 kDa subunit; FACTp80; Facilitates chromatin transcription complex subunit SSRP1; Recombination signal sequence recognition protein 1; Structure-specific recognition protein 1; hSSRP1; T160
UniProt Gene Name:	SSRP1
UniProt Entry Name:	SSRP1_HUMAN

	Western blot analysis of extracts of various cell lines, using SSRP1 antibody (CAB6413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse liver using SSRP1 antibody (CAB6413) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of U2OS cells using SSRP1 antibody (CAB6413).

Antibodies

Anti-SSRP1 Antibody (CAB13636)

SSRP1 Antibody (PACO12479)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)