Name: Anti-SSR1 Antibody (CAB4129)
Brand: Cell Biology Antibodies 9
SKU: CAB4129
Availability: OutOfStock

Antibody Name:	Anti-SSR1 Antibody
Antibody SKU:	CAB4129
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-207 of human SSR1 (NP_003135.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	LO2, HeLa, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-207 of human SSR1 (NP_003135.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VLFR GGPR GLLA VAQD LTED EETV EDSI IEDE DDEA EVEE DEPT DLVE DKEE EDVS GEPE ASPS ADTT ILFV KGED FPAN NIVK FLVG FTNK GTED FIVE SLDA SFRY PQDY QFYI QNFT ALPL NTVV PPQR QATF EYSF IPAE PMGG RPFG LVIN LNYK DLNG NVFQ DAVF NQTV TVIE REDG LDGE T
Gene ID:	6745
Uniprot:	P43307
Cellular Location:	Endoplasmic reticulum membrane, Single-pass type I membrane protein
Calculated MW:	29kDa/32kDa
Observed MW:	37kDa

Synonyms:

SSR1, TRAPA

Background:

The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	SSR1: TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins. Belongs to the TRAP-alpha family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p24.3 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane Molecular Function:protein binding Biological Process: cotranslational protein targeting to membrane; positive regulation of cell proliferation
NCBI Summary:	The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
UniProt Code:	P43307
NCBI GenInfo Identifier:	22261821
NCBI Gene ID:	6745
NCBI Accession:	P43307.3
UniProt Secondary Accession:	P43307,Q53GX2, Q53H19, Q5TAM3, Q6IB43, Q8NBH9, Q96IA2 Q9TNQ8, Q9UN49, A8K685,
UniProt Related Accession:	P43307
Molecular Weight:	29,373 Da
NCBI Full Name:	Translocon-associated protein subunit alpha
NCBI Synonym Full Names:	signal sequence receptor subunit 1
NCBI Official Symbol:	SSR1
NCBI Official Synonym Symbols:	TRAPA
NCBI Protein Information:	translocon-associated protein subunit alpha
UniProt Protein Name:	Translocon-associated protein subunit alpha
UniProt Synonym Protein Names:	Signal sequence receptor subunit alpha; SSR-alpha
Protein Family:	SWI/SNF and RSC complexes
UniProt Gene Name:	SSR1
UniProt Entry Name:	SSRA_HUMAN

Western blot analysis of extracts of various cell lines, using SSR1 Antibody (CAB4129) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.

Antibodies

SSR1 Antibody (PACO12476)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)