Cell Biology Antibodies 2

Anti-SSPN Antibody (CAB12191)

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SKU:
CAB12191
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SSPN Antibody
Antibody SKU:CAB12191
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-60 of human SSPN (NP_005077.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:NIH/3T3, Mouse skeletal muscle, Mouse heart, Mouse brain, Rat heart, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-60 of human SSPN (NP_005077.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGKN KQPR GQQR QGGP PAAD AAGP DDME PKKG TGAP KECG EEEP RTCC GCRF PLLL ALLQ
Gene ID:8082
Uniprot:Q14714
Cellular Location:Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, sarcolemma, synapse
Calculated MW:14kDa/15kDa/26kDa
Observed MW:27kDa
Synonyms:SSPN, DAGA5, KRAG, NSPN, SPN1, SPN2, sarcospan
Background:This gene encodes a member of the dystrophin-glycoprotein complex (DGC). The DGC spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Two alternatively spliced transcript variants that encode different protein isoforms have been described.
UniProt Protein Function:SSPN: Component of the dystrophin-glycoprotein complex (DGC), a complex that spans the muscle plasma membrane and forms a link between the F-actin cytoskeleton and the extracellular matrix. Preferentially associates with the sarcoglycan subcomplex of the DGC. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: transport vesicle

Biological Process: cell adhesion; muscle contraction

NCBI Summary:This gene encodes a member of the dystrophin-glycoprotein complex (DGC). The DGC spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Two alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Oct 2008]
UniProt Code:Q14714
NCBI GenInfo Identifier:13431900
NCBI Gene ID:8082
NCBI Accession:Q14714.3
UniProt Secondary Accession:Q14714,B3KS67,
UniProt Related Accession:Q14714
Molecular Weight:27kDa
NCBI Full Name:Sarcospan
NCBI Synonym Full Names:sarcospan
NCBI Official Symbol:SSPN  
NCBI Official Synonym Symbols:KRAG; NSPN; SPN1; SPN2; DAGA5  
NCBI Protein Information:sarcospan
UniProt Protein Name:Sarcospan
UniProt Synonym Protein Names:K-ras oncogene-associated protein; Kirsten-ras-associated protein
Protein Family:Sarcospan
UniProt Gene Name:SSPN  
Anti-SSPN Antibody (CAB12191)Western blot analysis of extracts of various cell lines, using SSPN antibody (CAB12191) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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