Antibody Name:	Anti-SRSF7 Antibody
Antibody SKU:	CAB16738
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-120 of human SRSF7 (NP_001026854).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse brain, Mouse kidney, Rat brain, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-120 of human SRSF7 (NP_001026854).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AEDA VRGL DGKV ICGS RVRV ELST GMPR RSRF DRPP ARRP FDPN DRCY ECGE KGHY AYDC H
Gene ID:	6432
Uniprot:	Q16629
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	15kDa/26kDa/27kDa
Observed MW:	35KDa

Synonyms:

SRSF7, 9G8, AAG3, SFRS7

Background:

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	SFRS7: Required for pre-mRNA splicing. Can also modulate alternative splicing in vitro. Represses the splicing of MAPT/Tau exon 10. Belongs to the splicing factor SR family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA splicing; Spliceosome; RNA-binding Chromosomal Location of Human Ortholog: 2p22.1 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:protein binding; zinc ion binding; nucleotide binding Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; mRNA export from nucleus; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription
NCBI Summary:	The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]
UniProt Code:	Q16629
NCBI GenInfo Identifier:	3929380
NCBI Gene ID:	6432
NCBI Accession:	Q16629.1
UniProt Secondary Accession:	Q16629,Q564D3, B4DLU6, G5E9M3,
UniProt Related Accession:	Q16629
Molecular Weight:	238
NCBI Full Name:	Serine/arginine-rich splicing factor 7
NCBI Synonym Full Names:	serine/arginine-rich splicing factor 7
NCBI Official Symbol:	SRSF7
NCBI Official Synonym Symbols:	9G8; AAG3; SFRS7
NCBI Protein Information:	serine/arginine-rich splicing factor 7; splicing factor 9G8; SR splicing factor 7; aging-associated protein 3; splicing factor, arginine/serine-rich 7, 35kDa
UniProt Protein Name:	Serine/arginine-rich splicing factor 7
UniProt Synonym Protein Names:	Splicing factor 9G8; Splicing factor, arginine/serine-rich 7
Protein Family:	Serine/arginine-rich splicing factor
UniProt Gene Name:	SRSF7
UniProt Entry Name:	SRSF7_HUMAN

	Western blot analysis of extracts of various cell lines, using SRSF7 Rabbit pAb (CAB16738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded mouse testis using SRSF7 Rabbit pAb (CAB16738) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal using SRSF7 Rabbit pAb (CAB16738) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using SRSF7 antibody (CAB16738) at dilution of 1:100. Blue: DAPI for nuclear staining.