Antibody Name:	Anti-SRSF2 Antibody
Antibody SKU:	CAB3635
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-75 of human SRSF2 (NP_003007.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse lung, Raji, THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-75 of human SRSF2 (NP_003007.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSYG RPPP DVEG MTSL KVDN LTYR TSPD TLRR VFEK YGRV GDVY IPRD RYTK ESRG FAFV RFHD KRDA EDAM DAM
Gene ID:	6427
Uniprot:	Q01130
Cellular Location:	Nucleus, Nucleus speckle, nucleoplasm
Calculated MW:	24kDa/25kDa
Observed MW:	28kDa/32kDa

Synonyms:

SRSF2, PR264, SC-35, SC35, SFRS2, SFRS2A, SRp30b

Background:

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 11.

UniProt Protein Function:	SFRS2: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre- mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment. Belongs to the splicing factor SR family.
UniProt Protein Details:	Protein type:RNA processing; RNA splicing; Spliceosome Chromosomal Location of Human Ortholog: 17q25.1 Cellular Component: nucleoplasm; spliceosome; PML body; cytoplasm; nuclear speck; nucleus Molecular Function:protein binding; nucleotide binding; transcription corepressor activity Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription
NCBI Summary:	The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 11. [provided by RefSeq, Sep 2010]
UniProt Code:	Q01130
NCBI GenInfo Identifier:	60416437
NCBI Gene ID:	6427
NCBI Accession:	Q01130.4
UniProt Secondary Accession:	Q01130,Q62093, Q6PDU1,
UniProt Related Accession:	Q01130
Molecular Weight:	Observed MW: 28kDa/32kDaCalculated MW: 24kDa/25kDa
NCBI Full Name:	Serine/arginine-rich splicing factor 2
NCBI Synonym Full Names:	serine/arginine-rich splicing factor 2
NCBI Official Symbol:	SRSF2
NCBI Official Synonym Symbols:	SC35; PR264; SC-35; SFRS2; SFRS2A; SRp30b
NCBI Protein Information:	serine/arginine-rich splicing factor 2; SR splicing factor 2; splicing factor SC35; splicing component, 35 kDa; splicing factor, arginine/serine-rich 2
UniProt Protein Name:	Serine/arginine-rich splicing factor 2
UniProt Synonym Protein Names:	Protein PR264; Splicing component, 35 kDa; Splicing factor SC35; SC-35; Splicing factor, arginine/serine-rich 2
Protein Family:	Serine/arginine-rich splicing factor
UniProt Gene Name:	SRSF2
UniProt Entry Name:	SRSF2_HUMAN

	Western blot analysis of extracts of mouse lung, using SRSF2 antibody (CAB3635) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Western blot analysis of extracts of various cell lines, using SRSF2 antibody (CAB3635) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded Rat lung using SRSF2 antibody (CAB3635) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human lung using SRSF2 antibody (CAB3635) at dilution of 1:100 (40x lens).