Antibody Name:	Anti-SRP72 Antibody (CAB10899)
Antibody SKU:	CAB10899
Antibody Size:	50µL, 100µL
Application:	Western blotting, Immunohistochemistry
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 440-544 of human SRP72 (NP_008878.3).

Application:	Western blotting, Immunohistochemistry
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	HeLa, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 440-544 of human SRP72 (NP_008878.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QPKS PAHL SLIR EAAN FKLK YGRK KEAI SDLQ QLWK QNPK DIHT LAQL ISAY SLVD PEKA KALS KHLP SSDS MSLK VDVE ALEN SAGA TYIR KKGG KVTG DSQP K
Cellular Location:	Cytoplasm, Endoplasmic reticulum
Calculated MW:	67kDa/74kDa
Observed MW:	72KDa

Synonyms:

SRP72, BMFF, BMFS1, HEL103

Background:

This gene encodes the 72 kDa subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates the targeting of secretory proteins to the endoplasmic reticulum (ER). The SRP complex consists of a 7S RNA and 6 protein subunits: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72, that are bound to the 7S RNA as monomers or heterodimers. SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition, translational arrest, and ER membrane targeting by interaction with the docking protein. Mutations in this gene are associated with familial bone marrow failure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

	Immunohistochemistry of paraffin-embedded rat brain using SRP72 Rabbit pAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using SRP72 Rabbit pAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded human esophageal cancer using SRP72 Rabbit pAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Western blot analysis of extracts of various cell lines, using SRP72 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.