Name: Anti-SRI Antibody (CAB19921)[KO Validated]
Brand: KO Validated Antibodies 2
SKU: CAB19921
Availability: OutOfStock

Antibody Name:	Anti-SRI Antibody [KO Validated]
Antibody SKU:	CAB19921
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human SRI.

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant protein of human SRI.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	6717
Uniprot:	P30626
Cellular Location:	Cytoplasm, Cytoplasmic side, Peripheral membrane protein, Sarcoplasmic reticulum membrane
Calculated MW:	20kDa/21kDa
Observed MW:	18KDa

Anti-SRI Antibody [KO Validated] (CAB19921)

Western blot analysis of extracts from normal (control) and SRI knockout (KO) HeLa cells, using SRI antibody (CAB19921) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Synonyms:

SRI, CP-22, CP22, SCN, V19, sorcin

Background:

This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene.

UniProt Protein Function:	SRI: Calcium-binding protein that modulates excitation- contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels.
UniProt Protein Details:	Protein type:Calcium-binding Chromosomal Location of Human Ortholog: 7q21.1 Cellular Component: nucleoplasm; endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; smooth endoplasmic reticulum; membrane; mitochondrion; sarcoplasmic reticulum; T-tubule; cytoplasm; nerve terminal; cytosol; Z disc Molecular Function:protein binding; protease binding; protein heterodimerization activity; calcium channel regulator activity; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; receptor binding Biological Process: regulation of action potential; cytoplasmic sequestering of transcription factor; muscle development; negative regulation of heart rate; heart development; regulation of striated muscle contraction; regulation of calcium ion transport; proteolysis; signal transduction; regulation of heart contraction; transport; calcium ion transport; positive regulation of release of sequestered calcium ion into cytosol; intracellular sequestering of iron ion
NCBI Summary:	This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene. [provided by RefSeq, Mar 2012]
UniProt Code:	P30626
NCBI GenInfo Identifier:	267021
NCBI Gene ID:	6717
NCBI Accession:	P30626.1
UniProt Secondary Accession:	P30626,A8MTH6, B4DKK2, D6W5Q0,
UniProt Related Accession:	P30626
Molecular Weight:	198
NCBI Full Name:	Sorcin
NCBI Synonym Full Names:	sorcin
NCBI Official Symbol:	SRI
NCBI Official Synonym Symbols:	SCN; V19; CP22; CP-22
NCBI Protein Information:	sorcin; H_RG167B05.1; 22 kDa protein; calcium binding protein amplified in mutlidrug-resistant cells
UniProt Protein Name:	Sorcin
UniProt Synonym Protein Names:	22 kDa protein; CP-22; CP22; V19
UniProt Gene Name:	SRI
UniProt Entry Name:	SORCN_HUMAN