Name: Anti-SRA1 Antibody (CAB16296)
Brand: Cell Death Antibodies 1
SKU: CAB16296
Availability: OutOfStock

Antibody Name:	Anti-SRA1 Antibody
Antibody SKU:	CAB16296
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SRA1 (NP_001030312.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-431, Mouse testis, Rat liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SRA1 (NP_001030312.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTRC PAGQ AEVE MAEL YVKP GNKE RGWN DPPQ FSYG LQTQ AGGP RRSL LTKR VAAP QDGS PRVP ASET SPGP PPMG PPPP SSKA PRSP PVGS GPAS GVEP
Gene ID:	10011
Uniprot:	Q9HD15
Cellular Location:
Calculated MW:	25kDa
Observed MW:	26kDa, 37kDa

Synonyms:

SRA1, SRA, SRAP, STRAA1, pp7684

Background:

Both long non-coding and protein-coding RNAs are transcribed from this gene, and they represent alternatively spliced transcript variants. This gene was initially defined as a non-coding RNA, which is a coactivator for several nuclear receptors (NRs) and is associated with breast cancer. It has now been found that this gene is involved in the regulation of many NR and non-NR activities, including metabolism, adipogenesis and chromatin organization. The long non-coding RNA transcripts interact with a variety of proteins, including the protein encoded by this gene. The encoded protein acts as a transcriptional repressor by binding to the non-coding RNA.

UniProt Protein Function:	SRA1: Functional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand- dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis. Belongs to the SRA1 family.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 5q31.3 Cellular Component: nucleoplasm; microtubule cytoskeleton; intercellular bridge; cytoplasm; plasma membrane; nucleus; ribonucleoprotein complex Molecular Function:protein binding; ligand-dependent nuclear receptor transcription coactivator activity; transcription coactivator activity Biological Process: regulation of apoptosis; cell proliferation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; apoptosis; cell differentiation
NCBI Summary:	Both long non-coding and protein-coding RNAs are transcribed from this gene, and they represent alternatively spliced transcript variants. This gene was initially defined as a non-coding RNA, which is a coactivator for several nuclear receptors (NRs) and is associated with breast cancer. It has now been found that this gene is involved in the regulation of many NR and non-NR activities, including metabolism, adipogenesis and chromatin organization. The long non-coding RNA transcripts interact with a variety of proteins, including the protein encoded by this gene. The encoded protein acts as a transcriptional repressor by binding to the non-coding RNA. [provided by RefSeq, Mar 2012]
UniProt Code:	Q9HD15
NCBI GenInfo Identifier:	74718904
NCBI Gene ID:	10011
NCBI Accession:	Q9HD15.1
UniProt Secondary Accession:	Q9HD15,Q6NVU9, Q8IXM1, Q9HD13, Q9HD14,
UniProt Related Accession:	Q9HD15
Molecular Weight:	25,673 Da
NCBI Full Name:	Steroid receptor RNA activator 1
NCBI Synonym Full Names:	steroid receptor RNA activator 1
NCBI Official Symbol:	SRA1
NCBI Official Synonym Symbols:	SRA; SRAP; STRAA1; pp7684
NCBI Protein Information:	steroid receptor RNA activator 1; SRA1; steroid receptor coactivator; steroid receptor RNA activator protein; steroid receptor RNA activator 1 (complexes with NCOA1)
UniProt Protein Name:	Steroid receptor RNA activator 1
UniProt Synonym Protein Names:	Steroid receptor RNA activator protein; SRAP
Protein Family:	Steroid receptor RNA activator
UniProt Gene Name:	SRA1
UniProt Entry Name:	SRA1_HUMAN

Western blot analysis of extracts of various cell lines, using SRA1 antibody (CAB16296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.

Antibodies

SRA1 Antibody (PACO12452)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)