Antibody Name:	Anti-SQSTM1 / p62 Antibody [KO Validated]
Antibody SKU:	CAB11483
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1 / p62 (NP_001135770.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, U-87MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1 / p62 (NP_001135770.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAMS YVKD DIFR IYIK EKKE CRRD HRPP CAQE APRN MVHP NVIC DGCN GPVV GTRY KCSV CPDY DLCS VCEG KGLH RGHT KLAF PSPF GHLS EGFS HSRW LRKV KHGH FGWP GWEM GPPG NWSP RPPR AGEA RPGP TAES ASGP SEDP SVNF LKNV GESV AAAL SPLG IEVD IDVE HGGK RSRL TPVS PESS STEE KSSS QPSS CCSD PSKP GGNV EGAT QSLA EQMR KIAL ESEG RPEE QMES DNCS GGDD DWTH LSSK EVDP STGE LQSL QMPE SEGP SSLD PSQE GPTG LKEA ALYP HLPP EADP RLIE SLSQ MLSM GFSD EGGW LTRL LQTK NYDI GAAL DTIQ YSKH PPPL
Gene ID:	8878
Uniprot:	Q13501
Cellular Location:	Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome
Calculated MW:	38kDa/47kDa
Observed MW:	62kDa

Synonyms:

SQSTM1, A170, DMRV, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B

Background:

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

UniProt Protein Function:	SQSTM1: an adapter protein which binds ubiquitin, shuttling proteins to the proteasome. Co-localizes along with the E3 ubiquitin ligase, TRAF6, to aggregates from Alzheimer's disease brains but not in control brain. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Forms ternary complexes with PKCZ and Kv-beta2 or PKCZ and GABBR3. Also interacts with KCNAB1, GABRR1, GABRR2 and GABRR3. Interacts with EBI3, LCK, RASA1, PKCZ , PKCI, NR2F2, NTRK1, NTRK2, NTRK3, NBR1, MEK5, TRIM55 and MAPKAPK5. Interacts with the proteasome subunits PSMD4 and PSMC2. Interacts with K63- polyubiquitinated MAPT/TAU. Interacts with IKBKB through PRKCZ and PRKCI. Interacts with NGFR through TRAF6 and bridges that complex to NTRK1. Forms a complex with MEK5 and PRKCZ or PRKCI. Component of a ternary complex with PAWR and PRKCZ. Upon TNF-alpha stimulation, interacts with RIPK1 problably bridging IKBKB to the TNF-R1 complex composed of TNF-R1/TNFRSF1A, TRADD and RIPK1. Forms a complex with JUB/Ajuba, PRKCZ and TRAF6. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Autophagy; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 5q35 Cellular Component: nucleoplasm; PML body; endoplasmic reticulum; lysosome; cytoplasm; late endosome; autophagic vacuole; cytoplasmic vesicle; inclusion body; cytosol Molecular Function:identical protein binding; protein serine/threonine kinase activity; protein binding; ubiquitin binding; protein homodimerization activity; protein kinase C binding; zinc ion binding; SH2 domain binding; receptor tyrosine kinase binding; protein kinase binding Biological Process: regulation of Ras protein signal transduction; ubiquitin-dependent protein catabolic process; nerve growth factor receptor signaling pathway; immune system process; positive regulation of apoptosis; protein heterooligomerization; macroautophagy; endosome transport; protein amino acid phosphorylation; protein localization; response to stress; autophagy; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; cell differentiation; regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of macroautophagy; negative regulation of apoptosis Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 3; Paget Disease Of Bone
NCBI Summary:	This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
UniProt Code:	Q13501
NCBI GenInfo Identifier:	74735628
NCBI Gene ID:	8878
NCBI Accession:	Q13501.1
UniProt Related Accession:	Q13501
Molecular Weight:
NCBI Full Name:	Sequestosome-1
NCBI Synonym Full Names:	sequestosome 1
NCBI Official Symbol:	SQSTM1
NCBI Official Synonym Symbols:	p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
NCBI Protein Information:	sequestosome-1
UniProt Protein Name:	Sequestosome-1
UniProt Synonym Protein Names:	EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62
UniProt Gene Name:	SQSTM1
UniProt Entry Name:	SQSTM_HUMAN

	Western blot analysis of extracts of various cell lines, using SQSTM1 antibody (CAB11483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts from normal (control) and SQSTM1 / p62 knockout (KO) 293T cells, using SQSTM1 / p62 antibody (CAB11483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded Rat ovary using SQSTM1 / p62 antibody (CAB11483) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human appendix using SQSTM1 / p62 antibody (CAB11483) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse liver using SQSTM1 / p62 antibody (CAB11483) at dilution of 1:100 (40x lens).