Cell Biology Antibodies 8

Anti-SQLE Antibody (CAB2428)

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SKU:
CAB2428
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SQLE Antibody
Antibody SKU:CAB2428
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 143-400 of human SQLE (NP_003120.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:SKOV3, Mouse liver, Mouse kidney, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 143-400 of human SQLE (NP_003120.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SRDG RKVT VIER DLKE PDRI VGEF LQPG GYHV LKDL GLGD TVEG LDAQ VVNG YMIH DQES KSEV QIPY PLSE NNQV QSGR AFHH GRFI MSLR KAAM AEPN AKFI EGVV LQLL EEDD VVMG VQYK DKET GDIK ELHA PLTV VADG LFSK FRKS LVSN KVSV SSHF VGFL MKNA PQFK ANHA ELIL ANPS PVLI YQIS SSET RVLV DIRG EMPR NLRE YMVE KIYP QIPD HLKE PFLE ATDN SHLR SMPA SFLP PSSV KK
Gene ID:6713
Uniprot:Q14534
Cellular Location:Endoplasmic reticulum membrane, Microsome membrane, Multi-pass membrane protein
Calculated MW:63kDa
Observed MW:71kDa
Synonyms:SQLE
Background:Squalene epoxidase catalyzes the first oxygenation step in sterol biosynthesis and is thought to be one of the rate-limiting enzymes in this pathway.
UniProt Protein Function:SQLE: Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway. Belongs to the squalene monooxygenase family.
UniProt Protein Details:

Protein type:Lipid Metabolism - steroid biosynthesis; EC 1.14.13.132; Oxidoreductase; Endoplasmic reticulum; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q24.1

Cellular Component: endoplasmic reticulum membrane

Molecular Function:squalene monooxygenase activity

Biological Process: cholesterol biosynthetic process

NCBI Summary:Squalene epoxidase catalyzes the first oxygenation step in sterol biosynthesis and is thought to be one of the rate-limiting enzymes in this pathway. [provided by RefSeq, Jul 2008]
UniProt Code:Q14534
NCBI GenInfo Identifier:296439362
NCBI Gene ID:6713
NCBI Accession:Q14534.3
UniProt Secondary Accession:Q14534,Q9UEK6,
UniProt Related Accession:Q14534
Molecular Weight:63,923 Da
NCBI Full Name:Squalene monooxygenase
NCBI Synonym Full Names:squalene epoxidase
NCBI Official Symbol:SQLE  
NCBI Protein Information:squalene monooxygenase
UniProt Protein Name:Squalene monooxygenase
UniProt Synonym Protein Names:Squalene epoxidase; SE
Protein Family:Squalene monooxygenase
UniProt Gene Name:SQLE  
UniProt Entry Name:ERG1_HUMAN
Anti-SQLE Antibody (CAB2428)Western blot analysis of extracts of various cell lines, using SQLE antibody (CAB2428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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