Cell Biology Antibodies 10

Anti-SPRY4 Antibody (CAB6263)

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SKU:
CAB6263
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SPRY4 Antibody
Antibody SKU:CAB6263
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-210 of human SPRY4 (NP_112226.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, U-87MG, SW480, HepG2, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-210 of human SPRY4 (NP_112226.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QSAP LTPN SVMV QPLL DSRM SHSR LQHP LTIL PIDQ VKTS HVEN DYID NPSL ALTT GPKR TRGG APEL APTP ARCD QDVT HHWI SFSG RPSS VSSS SSTS SDQR LLDH MAPP PVAD QASP RAVR IQPK VVHC QPLD LKGP AVPP ELDK HFLL CEAC GKCK CKEC ASPR TLPS CWVC NQEC L
Gene ID:81848
Uniprot:Q9C004
Cellular Location:Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, ruffle membrane
Calculated MW:11kDa/32kDa
Observed MW:37kDa
Synonyms:SPRY4, HH17
Background:This gene encodes a member of a family of cysteine- and proline-rich proteins. The encoded protein is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. Activity of this protein impairs the formation of active GTP-RAS. Nucleotide variation in this gene has been associated with hypogonadotropic hypogonadism 17 with or without anosmia. Alternative splicing results in a multiple transcript variants.
UniProt Protein Function:SPRY4: Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1. Belongs to the sprouty family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Inhibitor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: cytoplasm; focal adhesion; Golgi apparatus

Molecular Function:protein binding

Disease: Hypogonadotropic Hypogonadism 17 With Or Without Anosmia

NCBI Summary:This gene encodes a member of a family of cysteine- and proline-rich proteins. The encoded protein is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. Activity of this protein impairs the formation of active GTP-RAS. Nucleotide variation in this gene has been associated with hypogonadotropic hypogonadism 17 with or without anosmia. Alternative splicing results in a multiple transcript variants. [provided by RefSeq, Jun 2014]
UniProt Code:Q9C004
NCBI GenInfo Identifier:14916719
NCBI Gene ID:81848
NCBI Accession:Q9C004.2
UniProt Secondary Accession:Q9C004,Q6QIX2, Q9C003, A4FVB2, A4FVB3,
UniProt Related Accession:Q9C004
Molecular Weight:11,482 Da
NCBI Full Name:Protein sprouty homolog 4
NCBI Synonym Full Names:sprouty RTK signaling antagonist 4
NCBI Official Symbol:SPRY4  
NCBI Official Synonym Symbols:HH17  
NCBI Protein Information:protein sprouty homolog 4
UniProt Protein Name:Protein sprouty homolog 4
Protein Family:Protein sprouty
UniProt Gene Name:SPRY4  
UniProt Entry Name:SPY4_HUMAN
Anti-SPRY4 Antibody (CAB6263)Western blot analysis of extracts of various cell lines, using SPRY4 antibody (CAB6263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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