Cell Biology Antibodies 7
Anti-SPRY2 Antibody (CAB1855)
(No reviews yet)
Write a Review
- SKU:
- CAB1855
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SPRY2 Antibody |
| Antibody SKU: | CAB1855 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SPRY2 (NP_005833.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-251MG, Jurkat, Mouse heart |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SPRY2 (NP_005833.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEAR AQSG NGSQ PLLQ TPRD GGRQ RGEP DPRD ALTQ QVHV LSLD QIRA IRNT NEYT EGPT VVPR PGLK PAPR PSTQ HKHE RLHG LPEH RQPP RLQH SQVH |
| Gene ID: | 10253 |
| Uniprot: | O43597 |
| Cellular Location: | Cell projection, Cytoplasm, cytoskeleton, ruffle membrane |
| Calculated MW: | 34kDa |
| Observed MW: | 38kDa |
| Synonyms: | SPRY2, IGAN3, hSPRY2 |
| Background: | This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein. |
| UniProt Protein Function: | SPRY2: a feedback inhibitor of growth factor receptor signaling. Belongs to the sprouty family. May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor). |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Inhibitor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 13q31.1 Cellular Component: cytoskeleton; cytosol; membrane; nucleus; plasma membrane Molecular Function:protein binding; protein kinase binding; protein serine/threonine kinase activator activity; protein serine/threonine kinase inhibitor activity Biological Process: negative regulation of apoptosis; negative regulation of cell projection organization and biogenesis; negative regulation of epidermal growth factor receptor signaling pathway; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade |
| NCBI Summary: | This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43597 |
| NCBI GenInfo Identifier: | 13124551 |
| NCBI Gene ID: | 10253 |
| NCBI Accession: | O43597.1 |
| UniProt Secondary Accession: | O43597,Q5T6Z7, B2R9J9, |
| UniProt Related Accession: | O43597 |
| Molecular Weight: | 34,688 Da |
| NCBI Full Name: | Protein sprouty homolog 2 |
| NCBI Synonym Full Names: | sprouty RTK signaling antagonist 2 |
| NCBI Official Symbol: | SPRY2 |
| NCBI Official Synonym Symbols: | IGAN3; hSPRY2 |
| NCBI Protein Information: | protein sprouty homolog 2 |
| UniProt Protein Name: | Protein sprouty homolog 2 |
| Protein Family: | Protein sprouty |
| UniProt Gene Name: | SPRY2 |
| UniProt Entry Name: | SPY2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SPRY2 antibody (CAB1855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
View AllClose
Related Products
