Name: Anti-SPRTN Antibody (CAB11620)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB11620
Availability: OutOfStock

Antibody Name:	Anti-SPRTN Antibody
Antibody SKU:	CAB11620
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human SPRTN (NP_001010984.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human SPRTN (NP_001010984.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDDD LMLA LRLQ EEWN LQEA ERDH AQES LSLV DASW ELVD PTPD LQAL FVQF NDQF FWGQ LEAV EVKW SVRM TLCA GICS YEGK GGMC SIRL SEPL LKLR PRKD LVET LLHE MIHA YLFV TNND KDRE GHGP EFCK HMHR INSL TGAN ITVY HTFH DEVD EYRR HWWR CNGP CQHR PPYY GYVK RATN REPS AHDY WWAE HQKT CGGT YIKI KEPE NYSK KGKG KAKL GKEP VLAA ENKG
Gene ID:	83932
Uniprot:	Q9H040
Cellular Location:	Chromosome, Nucleus
Calculated MW:	24kDa/29kDa/55kDa
Observed MW:	60kDa

Synonyms:

SPRTN, C1orf124, DVC1, PRO4323, spartan

Background:

The protein encoded by this gene may play a role in DNA repair during replication of damaged DNA. This protein recruits valosin containing protein (p97) to stalled DNA replication forks where it may prevent excessive translesional DNA synthesis and limit the number of DNA-damage induced mutations. It may also be involved in replication-related G2/M-checkpoint regulation. Deficiency of a similar protein in mouse causes chromosomal instability and progeroid phenotypes. Mutations in this gene have been associated with Ruijs-Aalfs syndrome (RJALS). Alternatively spliced transcript variants have been identified.

UniProt Protein Function:	SPRTN: Regulator of UV-induced DNA damage response: acts as a 'reader' of ubiquitinated PCNA that enhances RAD18-mediated PCNA ubiquitination and translesion DNA synthesis. Recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. Also required for localization of DNA polymerase eta (POLH) to sites of UV damage. Belongs to the Spartan family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA repair, damage Chromosomal Location of Human Ortholog: 1q42.2 Cellular Component: nuclear speck; nucleoplasm; nucleus Molecular Function:protein binding; ubiquitin binding Biological Process: bypass DNA synthesis; positive regulation of protein ubiquitination; response to DNA damage stimulus; response to UV Disease: Ruijs-aalfs Syndrome
NCBI Summary:	The protein encoded by this gene may play a role in DNA repair during replication of damaged DNA. This protein recruits valosin containing protein (p97) to stalled DNA replication forks where it may prevent excessive translesional DNA synthesis and limit the number of DNA-damage induced mutations. It may also be involved in replication-related G2/M-checkpoint regulation. Deficiency of a similar protein in mouse causes chromosomal instability and progeroid phenotypes. Mutations in this gene have been associated with Ruijs-Aalfs syndrome (RJALS). Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]
UniProt Code:	Q9H040
NCBI GenInfo Identifier:	162416221
NCBI Gene ID:	83932
NCBI Accession:	Q9H040.2
UniProt Secondary Accession:	Q9H040,Q5TE78, Q6UWW6, Q96BC5, Q96KA0, B1AKT0, B5MEF7
UniProt Related Accession:	Q9H040
Molecular Weight:	55kDa
NCBI Full Name:	SprT-like domain-containing protein Spartan
NCBI Synonym Full Names:	SprT-like N-terminal domain
NCBI Official Symbol:	SPRTN
NCBI Official Synonym Symbols:	DVC1; PRO4323; spartan; C1orf124
NCBI Protein Information:	sprT-like domain-containing protein Spartan
UniProt Protein Name:	SprT-like domain-containing protein Spartan
UniProt Synonym Protein Names:	DNA damage protein targeting VCP; DVC1; Protein with SprT-like domain at the N terminus; Spartan
UniProt Gene Name:	SPRTN

Western blot analysis of extracts of U-87MG cells, using SPRTN antibody (CAB11620) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

Anti-SPRTN Antibody (CAB11663)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)