Signal Transduction Antibodies 2

Anti-SPRED1 Antibody (CAB14970)

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SKU:
CAB14970
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-SPRED1 Antibody
Antibody SKU:CAB14970
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 120-320 of human SPRED1 (NP_689807.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 120-320 of human SPRED1 (NP_689807.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IEDI SQGC PESK NEAE GADD LQAN EEDS SSSL VKDH LFQQ ETVV TSEP YRSS NIRP SPFE DLNA RRVY MQSQ ANQI TFGQ PGLD IQSR SMEY VQRQ ISKE CGSL KSQN RVPL KSIR HVSF QDED EIVR INPR DILI RRYA DYRH PDMW KNDL ERDD ADSS IQFS KPDS KKSD YLYS CGDE TKLS SPKD SVVF KTQP SSLK I
Gene ID:161742
Uniprot:Q7Z699
Cellular Location:Cell membrane, Membrane, Nucleus, Peripheral membrane protein, caveola
Calculated MW:50kDa
Observed MW:50kDa
Synonyms:SPRED1, NFLS, PPP1R147, hSpred1, spred-1
Background:The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS).
UniProt Protein Function:SPRED1: Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow. Defects in SPRED1 are the cause of neurofibromatosis type 1-like syndrome (NFLS). It is a disorder characterized mainly by cafe au lait macules without neurofibromas or other tumor manifestations of neurofibromatosis type 1, axillary freckling, and macrocephaly. Additional clinical manifestations include Noonan-like facial dysmorphism, lipomas, learning disabilities and attention deficit-hyperactivity.
UniProt Protein Details:

Protein type:Inhibitor

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: cytoplasm; cytosol; nucleoplasm; plasma membrane

Molecular Function:phosphatase binding; protein binding; protein kinase binding; protein serine/threonine kinase inhibitor activity; stem cell factor receptor binding

Biological Process: fibroblast growth factor receptor signaling pathway; inactivation of MAPK activity; MAPKKK cascade; positive regulation of DNA damage response, signal transduction by p53 class mediator

Disease: Legius Syndrome

NCBI Summary:The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq, Jul 2008]
UniProt Code:Q7Z699
NCBI GenInfo Identifier:57013078
NCBI Gene ID:161742
NCBI Accession:Q7Z699.2
UniProt Secondary Accession:Q7Z699,Q05D53, Q8N256, B2RPJ8,
UniProt Related Accession:Q7Z699
Molecular Weight:50,477 Da
NCBI Full Name:Sprouty-related, EVH1 domain-containing protein 1
NCBI Synonym Full Names:sprouty related EVH1 domain containing 1
NCBI Official Symbol:SPRED1  
NCBI Official Synonym Symbols:NFLS; hSpred1; spred-1; PPP1R147  
NCBI Protein Information:sprouty-related, EVH1 domain-containing protein 1
UniProt Protein Name:Sprouty-related, EVH1 domain-containing protein 1
Protein Family:Sprouty-related, EVH1 domain-containing protein
UniProt Gene Name:SPRED1  
UniProt Entry Name:SPRE1_HUMAN
Anti-SPRED1 Antibody (CAB14970)Western blot analysis of extracts of mouse brain, using SPRED1 antibody (CAB14970) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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