Signal Transduction Antibodies 1
Anti-SPP1 Antibody (CAB1361)
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- SKU:
- CAB1361
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-SPP1 Antibody |
| Antibody SKU: | CAB1361 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 17-314 of human SPP1 (NP_001035147.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HepG2, mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 17-314 of human SPP1 (NP_001035147.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IPVK QADS GSSE EKQL YNKY PDAV ATWL NPDP SQKQ NLLA PQNA VSSE ETND FKQE TLPS KSNE SHDH MDDM DDED DDDH VDSQ DSID SNDS DDVD DTDD SHQS DESH HSDE SDEL VTDF PTDL PATE VFTP VVPT VDTY DGRG DSVV YGLR SKSK KFRR PDIQ YPDA TDED ITSH MESE ELNG AYKA IPVA QDLN APSD WDSR GKDS YETS QLDD QSAE THSH KQSR LYKR KAND ESNE HSDV IDSQ ELSK VSRE FHSH EFHS HEDM LVVD PKSK EEDK HLKF RISH ELDS ASSE VN |
| Gene ID: | 6696 |
| Uniprot: | P10451 |
| Cellular Location: | Secreted |
| Calculated MW: | 32kDa/33kDa/35kDa |
| Observed MW: | 35kDa/66kDa |
| Synonyms: | BNSP, BSPI, ETA-1, OPN, Osteopontin, SPP1 |
| Background: | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 4q22.1 Cellular Component: extracellular space; cell projection; apical part of cell; perinuclear region of cytoplasm; extracellular region Molecular Function:protein binding; extracellular matrix binding; cytokine activity Biological Process: osteoblast differentiation; extracellular matrix disassembly; neutrophil chemotaxis; extracellular matrix organization and biogenesis; response to vitamin D; biomineral formation; response to steroid hormone stimulus; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; decidualization; cell adhesion; inflammatory response; embryo implantation |
| NCBI Summary: | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| UniProt Code: | P10451 |
| NCBI GenInfo Identifier: | 129260 |
| NCBI Gene ID: | 6696 |
| NCBI Accession: | P10451.1 |
| UniProt Secondary Accession: | P10451,Q15681, Q15682, Q15683, Q4W597, Q567T5, Q8NBK2 Q96IZ1, B2RDA1, |
| UniProt Related Accession: | P10451 |
| Molecular Weight: | 33,843 Da |
| NCBI Full Name: | Osteopontin |
| NCBI Synonym Full Names: | secreted phosphoprotein 1 |
| NCBI Official Symbol: | SPP1 |
| NCBI Official Synonym Symbols: | OPN; BNSP; BSPI; ETA-1 |
| NCBI Protein Information: | osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) |
| UniProt Protein Name: | Osteopontin |
| UniProt Synonym Protein Names: | Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin |
| Protein Family: | Osteopontin |
| UniProt Gene Name: | SPP1 |
| UniProt Entry Name: | OSTP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Osteopontin antibody (CAB1361) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
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