Signal Transduction Antibodies 1

Anti-SPOP Antibody (CAB12106)

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SKU:
CAB12106
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-SPOP Antibody
Antibody SKU:CAB12106
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 175-374 of human SPOP (NP_003554.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human, Mouse
Positive Samples:HeLa, HT-29
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 175-374 of human SPOP (NP_003554.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TMNM VKVP ECRL ADEL GGLW ENSR FTDC CLCV AGQE FQAH KAIL AARS PVFS AMFE HEME ESKK NRVE INDV EPEV FKEM MCFI YTGK APNL DKMA DDLL AAAD KYAL ERLK VMCE DALC SNLS VENA AEIL ILAD LHSA DQLK TQAV DFIN YHAS DVLE TSGW KSMV VSHP HLVA EAYR SLAS AQCP FLGP PRKR LKQS
Gene ID:8405
Uniprot:O43791
Cellular Location:Nucleus, Nucleus speckle
Calculated MW:42kDa
Observed MW:42kDa
Synonyms:SPOP, BTBD32, TEF2
Background:This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.
UniProt Protein Function:SPOP: Inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex. In complex with CUL3, involved in ubiquitination of BMI1, H2AFY and DAXX, and probably also in ubiquitination and proteasomal degradation of Gli2 or Gli3. Belongs to the Tdpoz family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: nucleoplasm; nucleus

Molecular Function:protein binding; ubiquitin protein ligase binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process

NCBI Summary:This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
UniProt Code:O43791
NCBI GenInfo Identifier:8134708
NCBI Gene ID:8405
NCBI Accession:O43791.1
UniProt Secondary Accession:O43791,Q53HJ1, B2R6S3, D3DTW7,
UniProt Related Accession:O43791
Molecular Weight:42,132 Da
NCBI Full Name:Speckle-type POZ protein
NCBI Synonym Full Names:speckle type BTB/POZ protein
NCBI Official Symbol:SPOP  
NCBI Official Synonym Symbols:TEF2; BTBD32  
NCBI Protein Information:speckle-type POZ protein
UniProt Protein Name:Speckle-type POZ protein
UniProt Synonym Protein Names:HIB homolog 1; Roadkill homolog 1
Protein Family:Speckle-type POZ protein
UniProt Gene Name:SPOP  
UniProt Entry Name:SPOP_HUMAN
Anti-SPOP Antibody (CAB12106)Western blot analysis of extracts of various cell lines, using SPOP antibody (CAB12106) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
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