Cell Biology Antibodies 5

Anti-SPON1 Antibody (CAB15130)

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SKU:
CAB15130
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SPON1 Antibody
Antibody SKU:CAB15130
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 29-300 of human SPON1 (NP_006099.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:mouse kidney, mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 29-300 of human SPON1 (NP_006099.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FSDE TLDK VPKS EGYC SRIL RAQG TRRE GYTE FSLR VEGD PDFY KPGT SYRV TLSA APPS YFRG FTLI ALRE NREG DKEE DHAG TFQI IDEE ETQF MSNC PVAV TEST PRRR TRIQ VFWI APPA GTGC VILK ASIV QKRI IYFQ DEGS LTKK LCEQ DSTF DGVT DKPI LDCC ACGT AKYR LTFY GNWS EKTH PKDY PRRA NHWS AIIG GSHS KNYV LWEY GGYA SEGV KQVA ELGS PVKM EEEI RQQS DEVL TVIK AKAQ WPAW QPLN VRAA
Gene ID:10418
Uniprot:Q9HCB6
Cellular Location:Secreted, extracellular matrix, extracellular space
Calculated MW:90kDa
Observed MW:91kDa
Synonyms:SPON1, VSGP/F-spondin, f-spondin, spondin-1
Background:
UniProt Protein Function:SPON1: Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: endoplasmic reticulum lumen; extracellular matrix; extracellular space

Molecular Function:protein binding

UniProt Code:Q9HCB6
NCBI GenInfo Identifier:52783472
NCBI Gene ID:10418
NCBI Accession:Q9HCB6.2
UniProt Secondary Accession:Q9HCB6,O94862, Q8NCD7, Q8WUR5, A8K6W5,
UniProt Related Accession:Q9HCB6
Molecular Weight:90,973 Da
NCBI Full Name:Spondin-1
NCBI Synonym Full Names:spondin 1
NCBI Official Symbol:SPON1  
NCBI Official Synonym Symbols:f-spondin; VSGP/F-spondin  
NCBI Protein Information:spondin-1
UniProt Protein Name:Spondin-1
UniProt Synonym Protein Names:F-spondin; Vascular smooth muscle cell growth-promoting factor
Protein Family:Spondin
UniProt Gene Name:SPON1  
UniProt Entry Name:SPON1_HUMAN
Anti-SPON1 Antibody (CAB15130)Western blot analysis of extracts of various cell lines, using SPON1 antibody (CAB15130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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