Name: Anti-SPK Antibody (CAB19947)[KO Validated]
Brand: KO Validated Antibodies 2
SKU: CAB19947
Availability: OutOfStock

Antibody Name:	Anti-SPK Antibody [KO Validated]
Antibody SKU:	CAB19947
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human SPK.

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant protein of human SPK.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	55872
Uniprot:	Q96KB5
Cellular Location:
Calculated MW:	36kDa/37kDa
Observed MW:	36KDa

Anti-SPK Antibody [KO Validated] (CAB19947)

Western blot analysis of extracts from normal (control) and SPK knockout (KO) 293T cells, using SPK antibody (CAB19947) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Synonyms:

PBK, CT84, HEL164, Nori-3, SPK, TOPK

Background:

This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	PBK: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. Interacts with DLG1 and TP53. Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. Activated by phosphorylation. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
UniProt Protein Details:	Protein type:EC 2.7.12.2; Cancer Testis Antigen (CTA); Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; Other group; TOPK family Chromosomal Location of Human Ortholog: 8p21.2 Cellular Component: nucleus Molecular Function:ATP binding; protein binding; protein serine/threonine kinase activity Biological Process: mitosis; negative regulation of inflammatory response; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of stress-activated MAPK cascade; protein amino acid phosphorylation
NCBI Summary:	This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Code:	Q96KB5
NCBI GenInfo Identifier:	83305809
NCBI Gene ID:	55872
NCBI Accession:	Q96KB5.3
UniProt Secondary Accession:	Q96KB5,Q9NPD9, Q9NYL7, Q9NZK6, B4DX68, D3DST2,
UniProt Related Accession:	Q96KB5
Molecular Weight:	37,211 Da
NCBI Full Name:	Lymphokine-activated killer T-cell-originated protein kinase
NCBI Synonym Full Names:	PDZ binding kinase
NCBI Official Symbol:	PBK
NCBI Official Synonym Symbols:	SPK; CT84; TOPK; HEL164; Nori-3
NCBI Protein Information:	lymphokine-activated killer T-cell-originated protein kinase
UniProt Protein Name:	Lymphokine-activated killer T-cell-originated protein kinase
UniProt Synonym Protein Names:	Cancer/testis antigen 84; CT84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase
Protein Family:	Lymphokine-activated killer T-cell-originated protein kinase
UniProt Gene Name:	PBK
UniProt Entry Name:	TOPK_HUMAN

Antibodies	ELISA Kits
Anti-SPK Antibody (CAB4852)	PBK/TOPK Colorimetric Cell-Based ELISA Kit
Phospho-PBK (T9) Antibody (PACO03013)	PBK/TOPK (Phospho-Thr9) Colorimetric Cell-Based ELISA Kit
PBK Antibody (PACO03014)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)