Cell Cycle Antibodies 2

Anti-SPICE1 Antibody (CAB7855)

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SKU:
CAB7855
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-SPICE1 Antibody
Antibody SKU:CAB7855
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SPICE1 (NP_653319.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HeLa, BT-474, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SPICE1 (NP_653319.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSFV RVNR CGPR VGVR KTPK VKKK KTSV KQEW DNTV TDLT VHRA TPED LVRR HEIH KSKN RALV HWEL QEKA LKRK WRKQ KPET LNLE KRRL SIMK EILS DQYQ MQDV LEKS DHLI AAAK ELFP RRRT GFPN VTVA PDSS QGPI VVNQ DPIT QSIF NESV IEPQ ALND VDGE EEGT VNSQ SGES ENEN ELDN SLNS QSNT NTDR FLQQ LTEE NFEL ISKL
Gene ID:152185
Uniprot:Q8N0Z3
Cellular Location:Cytoplasm, centriole, centrosome, cytoskeleton, microtubule organizing center, spindle
Calculated MW:96kDa
Observed MW:120kDa
Synonyms:SPICE1, CCDC52, SPICE
Background:
UniProt Protein Function:CCDC52: a protein of unknown function.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 3q13.2

Cellular Component: centriole; centrosome; spindle

Molecular Function:protein binding

Biological Process: metaphase plate congression; regulation of centriole replication

UniProt Code:Q8N0Z3
NCBI GenInfo Identifier:74728478
NCBI Gene ID:152185
NCBI Accession:Q8N0Z3.1
UniProt Secondary Accession:Q8N0Z3,Q8WUX6, D3DN72,
UniProt Related Accession:Q8N0Z3
Molecular Weight:96kDa
NCBI Full Name:Spindle and centriole-associated protein 1
NCBI Synonym Full Names:spindle and centriole associated protein 1
NCBI Official Symbol:SPICE1  
NCBI Official Synonym Symbols:SPICE; CCDC52  
NCBI Protein Information:spindle and centriole-associated protein 1
UniProt Protein Name:Spindle and centriole-associated protein 1
UniProt Synonym Protein Names:Coiled-coil domain-containing protein 52; Spindle and centriole-associated protein
Protein Family:Spindle and centriole-associated protein
UniProt Gene Name:SPICE1  
Anti-SPICE1 Antibody (CAB7855)Western blot analysis of extracts of various cell lines, using SPICE1 antibody (CAB7855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-SPICE1 Antibody (CAB7855)Immunohistochemistry of paraffin-embedded human stomach using SPICE1 antibody (CAB7855) at dilution of 1:100 (40x lens).
Anti-SPICE1 Antibody (CAB7855)Immunohistochemistry of paraffin-embedded rat brain using SPICE1 antibody (CAB7855) at dilution of 1:100 (40x lens).
Anti-SPICE1 Antibody (CAB7855)Immunohistochemistry of paraffin-embedded mouse kidney using SPICE1 antibody (CAB7855) at dilution of 1:100 (40x lens).
Anti-SPICE1 Antibody (CAB7855)Immunofluorescence analysis of U2OS cells using SPICE1 antibody (CAB7855) at dilution of 1:100. Blue: DAPI for nuclear staining.
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