Epigenetics & Nuclear Signaling Antibodies 4
Anti-SPIB Antibody (CAB7451)
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- SKU:
- CAB7451
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-SPIB Antibody |
| Antibody SKU: | CAB7451 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human SPIB (NP_003112.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human |
| Positive Samples: | Raji |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human SPIB (NP_003112.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLAL EAAQ LDGP HFSC LYPD GVFY DLDS CKHS SYPD SEGA PDSL WDWT VAPP VPAT PYEA FDPA AAAF SHPQ AAQL CYEP PTYS PAGN LELA PSLE APGP GLPA YPTE NFAS QTLV PPAY APYP SPVL SEEE DLPL DSPA LEVS DSES DEAL VAGP EGKG SEAG TRKK LRLY QFLL GLLT RGDM RECV WWVE PGAG VFQF SSKH KELL ARRW GQQK GNRK RMTY QKLA RALR NYAK TGEI RKVK RKLT YQFD SALL PAVR RA |
| Gene ID: | 6689 |
| Uniprot: | Q01892 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 18kDa/19kDa/28kDa |
| Observed MW: | 46kDa |
| Synonyms: | SPIB, SPI-B |
| Background: | The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | SPIB: Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 19q13.3-q13.4 Cellular Component: cytoplasm; nucleus Molecular Function:sequence-specific DNA binding Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; cell differentiation |
| NCBI Summary: | The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | Q01892 |
| NCBI GenInfo Identifier: | 548971 |
| NCBI Gene ID: | 6689 |
| NCBI Accession: | Q01892.1 |
| UniProt Secondary Accession: | Q01892,Q15359, A8K9C9, B4DUG6, |
| UniProt Related Accession: | Q01892 |
| Molecular Weight: | 262 |
| NCBI Full Name: | Transcription factor Spi-B |
| NCBI Synonym Full Names: | Spi-B transcription factor (Spi-1/PU.1 related) |
| NCBI Official Symbol: | SPIB |
| NCBI Official Synonym Symbols: | SPI-B |
| NCBI Protein Information: | transcription factor Spi-B |
| UniProt Protein Name: | Transcription factor Spi-B |
| Protein Family: | Transcription factor |
| UniProt Gene Name: | SPIB |
| UniProt Entry Name: | SPIB_HUMAN |
 | Western blot analysis of extracts of Raji cells, using SPIB antibody (CAB7451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of A-549 cells using SPIB antibody (CAB7451). Blue: DAPI for nuclear staining. |
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