Cell Biology Antibodies 8
Anti-SPHK2 Antibody (CAB3204)
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- SKU:
- CAB3204
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SPHK2 Antibody |
| Antibody SKU: | CAB3204 |
| Antibody Size: | 20uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human SPHK2 (NP_001191088.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human SPHK2 (NP_001191088.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MNGH LEAE EQQD QRPD QELT GSWG HGPR STLV RAKA MAPP PPPL AAST PLLH GEFG SYPA RGPR FALT LTSQ ALHI QRLR PKPE ARPR GGLV PLAE VSGC CTLR SRSP SDSA AYFC IYTY PRGR RGAR RRAT RTFR ADGA ATYE ENRA EAQR WATA LTCL LRGL P |
| Gene ID: | 56848 |
| Uniprot: | Q9NRA0 |
| Cellular Location: | Cytoplasm, Lysosome membrane, Membrane |
| Calculated MW: | 62kDa/65kDa/69kDa/80kDa |
| Observed MW: | 65kDa |
| Synonyms: | SPHK2, SK 2, SK-2, SPK 2, SPK-2, SK2, SPK2 |
| Background: | This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | SPHK2: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- dihydrosphingosine, D-erythro-sphingosine and L-threo- dihydrosphingosine. Binds phosphoinositides. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - sphingolipid; Kinase, lipid; EC 2.7.1.91 Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: membrane; lysosomal membrane; cytosol Molecular Function:D-erythro-sphingosine kinase activity; protein binding; Ras GTPase binding; sphinganine kinase activity; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity Biological Process: cell proliferation; blood vessel development; sphingolipid metabolic process; sphingolipid biosynthetic process; protein kinase C activation; positive regulation of cell proliferation; sphingosine metabolic process; sphinganine-1-phosphate biosynthetic process; brain development; lipid phosphorylation; negative regulation of apoptosis |
| NCBI Summary: | This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q9NRA0 |
| NCBI GenInfo Identifier: | 22001996 |
| NCBI Gene ID: | 56848 |
| NCBI Accession: | Q9NRA0.2 |
| UniProt Secondary Accession: | Q9NRA0,Q9BRN1, Q9H0Q2, Q9NWU7, A0T4C8, B4DU87, |
| UniProt Related Accession: | Q9NRA0 |
| Molecular Weight: | 654 |
| NCBI Full Name: | Sphingosine kinase 2 |
| NCBI Synonym Full Names: | sphingosine kinase 2 |
| NCBI Official Symbol: | SPHK2 |
| NCBI Official Synonym Symbols: | SK 2; SK-2; SPK 2; SPK-2 |
| NCBI Protein Information: | sphingosine kinase 2; sphingosine kinase type 2 |
| UniProt Protein Name: | Sphingosine kinase 2 |
| Protein Family: | Sphingosine kinase |
| UniProt Gene Name: | SPHK2 |
| UniProt Entry Name: | SPHK2_HUMAN |
 | Western blot analysis of extracts of mouse brain, using SPHK2 antibody (CAB3204). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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