Metabolism Antibodies 3

Anti-Spermidine synthase Antibody (CAB8151)

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SKU:
CAB8151
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:SRM Rabbit Polyclonal Antibody
Antibody SKU:CAB8151
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human SRM (NP_003123.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:22Rv1, HepG2, HeLa, Mouse liver, Mouse stomach, Mouse small intestine, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human SRM (NP_003123.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEPG PDGP AASG PAAI REGW FRET CSLW PGQA LSLQ VEQL LHHR RSRY QDIL VFRS KTYG NVLV LDGV IQCT ERDE FSYQ EMIA NLPL CSHP NPRK VLII GGGD GGVL REVV KHPS VESV VQCE IDED VIQV SKKF LPGM AIGY SSSK LTLH VGDG FEFM KQNQ DAFD VIIT DSSD PMGP AESL FKES YYQL MKTA LKED GVLC CQGE CQWL HLDL IKEM RQFC QSLF PVVA YAYC TIPT YPSG QIGF MLCS KNPS TNFQ EPVQ PLTQ QQVA QMQL KYYN SDVH RAAF VLPE FARK ALND VS
Gene ID:6723
Uniprot:P19623
Cellular Location:
Calculated MW:33kDa
Observed MW:35kDa
Synonyms:SRM, PAPT, SPDSY, SPS1, SRML1
Background:The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthase is one of four enzymes in the polyamine-biosynthetic pathway and carries out the final step of spermidine biosynthesis. This enzyme catalyzes the conversion of putrescine to spermidine using decarboxylated S-adenosylmethionine as the cofactor.
UniProt Protein Function:SRM: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM). Has a strong preference for putrescine as substrate, and has very low activity towards 1,3-diaminopropane. Has extremely low activity towards spermidine. Belongs to the spermidine/spermine synthase family.
UniProt Protein Details:

Protein type:Other Amino Acids Metabolism - glutathione; Amino Acid Metabolism - cysteine and methionine; Transferase; EC 2.5.1.16; Amino Acid Metabolism - arginine and proline; Other Amino Acids Metabolism - beta-alanine

Chromosomal Location of Human Ortholog: 1p36-p22

Cellular Component: cytosol

Molecular Function:protein binding; protein homodimerization activity; spermidine synthase activity

Biological Process: polyamine metabolic process; spermidine biosynthetic process

NCBI Summary:The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthase is one of four enzymes in the polyamine-biosynthetic pathway and carries out the final step of spermidine biosynthesis. This enzyme catalyzes the conversion of putrescine to spermidine using decarboxylated S-adenosylmethionine as the cofactor. [provided by RefSeq, Jul 2008]
UniProt Code:P19623
NCBI GenInfo Identifier:134811
NCBI Gene ID:6723
NCBI Accession:P19623.1
UniProt Secondary Accession:P19623,Q15511, B1AKP9,
UniProt Related Accession:P19623
Molecular Weight:33,825 Da
NCBI Full Name:Spermidine synthase
NCBI Synonym Full Names:spermidine synthase
NCBI Official Symbol:SRM  
NCBI Official Synonym Symbols:PAPT; SPS1; SPDSY; SRML1  
NCBI Protein Information:spermidine synthase
UniProt Protein Name:Spermidine synthase
UniProt Synonym Protein Names:Putrescine aminopropyltransferase
UniProt Gene Name:SRM  
UniProt Entry Name:SPEE_HUMAN
Anti-Spermidine synthase Polyclonal Antibody (CAB8151)Western blot analysis of extracts of various cell lines, using SRM antibody (CAB8151) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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