Cell Cycle Antibodies 1

Anti-SPDYA Antibody (CAB2153)

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SKU:
CAB2153
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-SPDYA Antibody
Antibody SKU:CAB2153
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human SPDYA (NP_877433.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, Mouse ovary, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human SPDYA (NP_877433.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MRHN QMCC ETPP TVTV YVKS GSNR SHQP KKPI TLKR PICK DNWQ AFEK NTHN NNKS KRPK GPCL VIQR QDMT AFFK LFDD DLIQ DFLW MDCC CKIA DKYL LAMT FVYF KRAK FTIS EHTR INFF IALY LANT VEED EEET KYEI FPWA LGKN WRKL FPNF LKLR DQLW DRID YRAI VSRR CCEE VMAI APTH YIWQ RERS VHHS GAVR NYNR DEVQ LPRG PSAT PVDC SLCG KKRR YVRL GLSS SSSL SSHT AGVT EKHS QDSY NSLS MDII GDPS QAYT GSEV VNDH QSNK GKKT NFLK KDKS MEWF TGSE E
Gene ID:245711
Uniprot:Q5MJ70
Cellular Location:Nucleus
Calculated MW:33kDa/36kDa
Observed MW:48kDa
Synonyms:SPDYA, RINGO3, RINGOA, SPDY1, SPY1
Background:
UniProt Protein Function:SPDYA: Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP1. Can activate CDK2 without promoting CDK2 phosphorylation. Mediates cell survival during the DNA damage process through activation of CDK2. Belongs to the Speedy/Ringo family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 2p23.2

Cellular Component: nucleus

Molecular Function:protein kinase binding

Biological Process: positive regulation of cyclin-dependent protein kinase activity; multicellular organismal development; positive regulation of cell proliferation; male meiosis; response to DNA damage stimulus; G1/S transition of mitotic cell cycle

UniProt Code:Q5MJ70
NCBI GenInfo Identifier:94730574
NCBI Gene ID:245711
NCBI Accession:Q5MJ70.2
UniProt Related Accession:Q5MJ70
Molecular Weight:33kDa; 36kDa
NCBI Full Name:Speedy protein A
NCBI Synonym Full Names:speedy/RINGO cell cycle regulator family member A
NCBI Official Symbol:SPDYA  
NCBI Official Synonym Symbols:SPY1; SPDY1; RINGO3; RINGOA  
NCBI Protein Information:speedy protein A
UniProt Protein Name:Speedy protein A
UniProt Synonym Protein Names:Rapid inducer of G2/M progression in oocytes A; RINGO A; hSpy/Ringo A; Speedy-1; Spy1
Protein Family:Speedy protein
UniProt Gene Name:SPDYA  
UniProt Entry Name:SPDYA_HUMAN
Anti-SPDYA Antibody (CAB2153)Western blot analysis of extracts of various cell lines, using SPDYA antibody (CAB2153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.
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