Cell Cycle Antibodies 1
Anti-SPDYA Antibody (CAB13607)
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- SKU:
- CAB13607
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-SPDYA Antibody |
| Antibody SKU: | CAB13607 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human SPDYA (NP_877433.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human SPDYA (NP_877433.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MRHN QMCC ETPP TVTV YVKS GSNR SHQP KKPI TLKR PICK DNWQ AFEK NTHN NNKS KRPK GPCL VIQR QDMT AFFK LFDD DLIQ DFLW MDCC CKIA DKYL LAMT FVYF KRAK FTIS EHTR INFF IALY LANT VEED EEET KYEI FPWA LGKN WRKL FPNF LKLR DQLW DRID YRAI VSRR CCEE VMAI APTH YIWQ RERS VHHS GAVR NYNR DEVQ LPRG PSAT PVDC SLCG KKRR YVRL GLSS SSSL SSHT AGVT EKHS QDSY NSLS MDII GDPS QAYT GSEV VNDH QSNK GKKT NFLK KDKS MEWF TGSE E |
| Gene ID: | 245711 |
| Uniprot: | Q5MJ70 |
| Cellular Location: | Nucleus |
| Calculated MW: | 33kDa/36kDa |
| Observed MW: | 50kDa |
| Synonyms: | SPDYA, RINGO3, RINGOA, SPDY1, SPY1 |
| Background: |
| UniProt Protein Function: | SPDYA: Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP1. Can activate CDK2 without promoting CDK2 phosphorylation. Mediates cell survival during the DNA damage process through activation of CDK2. Belongs to the Speedy/Ringo family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 2p23.2 Cellular Component: nucleus Molecular Function:protein kinase binding Biological Process: positive regulation of cyclin-dependent protein kinase activity; multicellular organismal development; positive regulation of cell proliferation; male meiosis; response to DNA damage stimulus; G1/S transition of mitotic cell cycle |
| UniProt Code: | Q5MJ70 |
| NCBI GenInfo Identifier: | 94730574 |
| NCBI Gene ID: | 245711 |
| NCBI Accession: | Q5MJ70.2 |
| UniProt Related Accession: | Q5MJ70 |
| Molecular Weight: | 33kDa; 36kDa |
| NCBI Full Name: | Speedy protein A |
| NCBI Synonym Full Names: | speedy/RINGO cell cycle regulator family member A |
| NCBI Official Symbol: | SPDYA |
| NCBI Official Synonym Symbols: | SPY1; SPDY1; RINGO3; RINGOA |
| NCBI Protein Information: | speedy protein A |
| UniProt Protein Name: | Speedy protein A |
| UniProt Synonym Protein Names: | Rapid inducer of G2/M progression in oocytes A; RINGO A; hSpy/Ringo A; Speedy-1; Spy1 |
| Protein Family: | Speedy protein |
| UniProt Gene Name: | SPDYA |
| UniProt Entry Name: | SPDYA_HUMAN |
 | Western blot analysis of extracts of A549 cells, using SPDYA antibody (CAB13607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s. |
 | Immunofluorescence analysis of MCF-7 cells using SPDYA antibody (CAB13607). Blue: DAPI for nuclear staining. |
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