Cell Biology Antibodies 5
Anti-SPARC Antibody (CAB14494)
- SKU:
- CAB14494
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SPARC Antibody |
| Antibody SKU: | CAB14494 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A375 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | APQQ EALP DETE VVEE TVAE VTEV SVGA NPVQ VEVG EFDD GAEE TEEE VVAE NPCQ NHHC KHGK VCEL DENN TPMC VCQD PTSC PAPI GEFE KVCS NDNK TFDS SCHF FATK CTLE GTKK GHKL HLDY IGPC KYIP PCLD SELT EFPL RMRD WLKN VLVT LYER DEDN NLLT EKQK LRVK KIHE NEKR LEAG DHPV ELLA RDFE KNYN MYIF PVHW QFGQ LDQH PIDG YLSH TELA PLRA PLIP MEHC TTRF FETC DLDN DKYI ALDE WAGC FGIK QKDI DKDL VI |
| Gene ID: | 6678 |
| Uniprot: | P09486 |
| Cellular Location: | Secreted, basement membrane, extracellular matrix, extracellular space |
| Calculated MW: | 34kDa |
| Observed MW: | 35kDa |
| Synonyms: | SPARC, BM-40, OI17 |
| Background: | This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 5q31.3-q32 Cellular Component: extracellular space; platelet alpha granule membrane; nuclear matrix; cell surface; mitochondrion; cytoplasm; plasma membrane; extracellular region; basement membrane Molecular Function:collagen binding; protein binding; extracellular matrix binding; calcium ion binding Biological Process: response to peptide hormone stimulus; receptor-mediated endocytosis; response to gravity; platelet activation; extracellular matrix organization and biogenesis; ossification; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; platelet degranulation; response to cadmium ion; response to ethanol; response to cytokine stimulus; response to lead ion; negative regulation of endothelial cell proliferation; regulation of cell morphogenesis; response to calcium ion; blood coagulation; inner ear development; lung development Disease: Osteogenesis Imperfecta, Type Xvii |
| NCBI Summary: | This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015] |
| UniProt Code: | P09486 |
| NCBI GenInfo Identifier: | 4507171 |
| NCBI Gene ID: | 6678 |
| NCBI Accession: | NP_003109 |
| UniProt Related Accession: | P09486 |
| Molecular Weight: | 35kDa |
| NCBI Full Name: | SPARC isoform 1 |
| NCBI Synonym Full Names: | secreted protein acidic and cysteine rich |
| NCBI Official Symbol: | SPARC |
| NCBI Official Synonym Symbols: | ON; OI17; BM-40 |
| NCBI Protein Information: | SPARC |
| UniProt Protein Name: | SPARC |
| UniProt Synonym Protein Names: | Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine |
| UniProt Gene Name: | SPARC |
| UniProt Entry Name: | SPRC_HUMAN |
 | Western blot analysis of extracts of A375 cells, using SPARC antibody (CAB14494) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of C6 cells using SPARC Polyclonal Antibody (CAB14494) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L-929 cells using SPARC Polyclonal Antibody (CAB14494) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using SPARC Polyclonal Antibody (CAB14494) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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