Cell Biology Antibodies 8

Anti-SPAM1 Antibody (CAB2120)

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SKU:
CAB2120
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SPAM1 Antibody
Antibody SKU:CAB2120
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:PC-3, Raji, BT-474
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PYID SITG VTVN GGIP QKIS LQDH LDKA KKDI TFYM PVDN LGMA VIDW EEWR PTWA RNWK PKDV YKNR SIEL VQQQ NVQL SLTE ATEK AKQE FEKA GKDF LVET IKLG KLLR PNHL WGYY LFPD CYNH HYKK PGYN GSCF NVEI KRND DLSW LWNE STAL YPSI YLNT QQSP VAAT LYVR NRVR EAIR VSKI PDAK SPLP V
Gene ID:6677
Uniprot:P38567
Cellular Location:Cell membrane, GPI-anchor, Lipid-anchor
Calculated MW:57kDa/58kDa
Observed MW:47kDa
Synonyms:SPAM1, HEL-S-96n, HYA1, HYAL1, HYAL3, HYAL5, PH-20, PH20, SPAG15
Background:Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:SPAM1: Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. Belongs to the glycosyl hydrolase 56 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Glycan Metabolism - glycosaminoglycan degradation; Hydrolase; EC 3.2.1.35; Membrane protein, GPI anchor; Cell surface

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: plasma membrane

Molecular Function:hyalurononglucosaminidase activity

Biological Process: sperm-egg recognition; fusion of sperm to egg plasma membrane; binding of sperm to zona pellucida; single fertilization; carbohydrate metabolic process; cell adhesion; multicellular organism reproduction

NCBI Summary:Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
UniProt Code:P38567
NCBI GenInfo Identifier:585673
NCBI Gene ID:6677
NCBI Accession:P38567.1
UniProt Secondary Accession:P38567,Q8TC30,
UniProt Related Accession:P38567
Molecular Weight:Calculated: 57kDa/58kDaObserved: 47kDa
NCBI Full Name:Hyaluronidase PH-20
NCBI Synonym Full Names:sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
NCBI Official Symbol:SPAM1  
NCBI Official Synonym Symbols:HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n  
NCBI Protein Information:hyaluronidase PH-20; hyal-PH20; sperm surface protein PH-20; hyaluronoglucosaminidase PH-20; epididymis secretory sperm binding protein Li 96n
UniProt Protein Name:Hyaluronidase PH-20
UniProt Synonym Protein Names:Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20
Protein Family:Hyaluronidase
UniProt Gene Name:SPAM1  
UniProt Entry Name:HYALP_HUMAN
Anti-SPAM1 Antibody (CAB2120)Western blot analysis of extracts of various cell lines, using SPAM1 antibody (CAB2120) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-SPAM1 Antibody (CAB2120)Immunohistochemistry of paraffin-embedded rat testis using SPAM1 Rabbit pAb (CAB2120) at dilution of 1:100 (40x lens).
Anti-SPAM1 Antibody (CAB2120)Immunofluorescence analysis of HeLa cells using SPAM1 antibody (CAB2120). Blue: DAPI for nuclear staining.
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