Name: Anti-SPAG6 Antibody (CAB3088)
Brand: Cell Biology Antibodies 8
SKU: CAB3088
Availability: OutOfStock

Antibody Name:	Anti-SPAG6 Antibody
Antibody SKU:	CAB3088
Antibody Size:	20uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 180-290 of human SPAG6 (NP_758442.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:1000
Reactivity:	Human
Positive Samples:	Jurkat, PC-3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 180-290 of human SPAG6 (NP_758442.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EIAL KRIA ASAL SDIA KHSP ELAQ TVVD AGAV AHLA QMIL NPDA KLKH QILS ALSQ VSKH SVDL AEMV VEAE IFPV VLTC LKDK DEYV KKNA STLI REIA KHTP ELSQ LVV
Gene ID:	9576
Uniprot:	O75602
Cellular Location:	Cell projection, Cytoplasm, cilium, cytoskeleton, flagellum
Calculated MW:	30kDa/49kDa/52kDa/55kDa/63kDa
Observed MW:	55kDa

Synonyms:

SPAG6, CT141, Repro-SA-1, pf16

Background:

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	SPAG6: Important for structural integrity of the central apparatus in the sperm tail and for flagellar motility. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell development/differentiation; Cancer Testis Antigen (CTA) Chromosomal Location of Human Ortholog: 10p12.2 Cellular Component: axoneme; microtubule cytoskeleton; nucleus Biological Process: spermatid development
NCBI Summary:	The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	O75602
NCBI GenInfo Identifier:	62901067
NCBI Gene ID:	9576
NCBI Accession:	O75602.1
UniProt Secondary Accession:	O75602,Q5VUX5, Q5VUX6, Q5VUX7, Q6FI74, Q8NHQ6, A8K1I8 B4DXZ4,
UniProt Related Accession:	O75602
Molecular Weight:	52,466 Da
NCBI Full Name:	Sperm-associated antigen 6
NCBI Synonym Full Names:	sperm associated antigen 6
NCBI Official Symbol:	SPAG6
NCBI Official Synonym Symbols:	pf16; CT141; Repro-SA-1
NCBI Protein Information:	sperm-associated antigen 6
UniProt Protein Name:	Sperm-associated antigen 6
UniProt Synonym Protein Names:	Protein PF16 homolog; Repro-SA-1; Sperm flagellar protein
Protein Family:	Sperm-associated antigen
UniProt Gene Name:	SPAG6
UniProt Entry Name:	SPAG6_HUMAN

Western blot analysis of extracts of various cell lines, using SPAG6 antibody (CAB3088). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Antibodies

SPAG6 Antibody (PACO12414)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)