Antibody Name:	Anti-SP3 Antibody
Antibody SKU:	CAB12790
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 240-350 of human SP3 (NP_003102.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, PC-3, Mouse liver, Mouse brain, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 240-350 of human SP3 (NP_003102.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AIGG SSFP GQTQ VVAN VPLG LPGN ITFV PINS VDLD SLGL SGSS QTMT AGIN ADGH LINT GQAM DSSD NSER TGER VSPD INET NTDT DLFV PTSS SSQL PVTI DSTG ILQ
Gene ID:	6670
Uniprot:	Q02447
Cellular Location:	Nucleus, PML body
Calculated MW:	52kDa/53kDa/75kDa/76kDa/80kDa/81kDa
Observed MW:	70kDa

Synonyms:

SP3, SPR2

Background:

This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.

UniProt Protein Function:	Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping.
NCBI Summary:	This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. [provided by RefSeq, Feb 2010]
UniProt Code:	Q02447
NCBI GenInfo Identifier:	30923147
NCBI Gene ID:	6670
NCBI Accession:	Q02447.3
UniProt Secondary Accession:	Q02447,Q69B26, Q69B27, Q8TD56, Q8WWU4, Q9BQR1, A0AVL9 B4E2B7,
UniProt Related Accession:	Q02447
Molecular Weight:	76,814 Da
NCBI Full Name:	Transcription factor Sp3
NCBI Synonym Full Names:	Sp3 transcription factor
NCBI Official Symbol:	SP3
NCBI Official Synonym Symbols:	SPR2
NCBI Protein Information:	transcription factor Sp3
UniProt Protein Name:	Transcription factor Sp3
UniProt Synonym Protein Names:	SPR-2
Protein Family:	Transcription factor
UniProt Gene Name:	SP3
UniProt Entry Name:	SP3_HUMAN

	Western blot analysis of extracts of various cell lines, using SP3 antibody (CAB12790) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human colon using SP3 Rabbit pAb (CAB12790) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat ovary using SP3 Rabbit pAb (CAB12790) at dilution of 1:100 (40x lens).