Immunology Antibodies 2

Anti-SP100 Antibody (CAB5851)

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SKU:
CAB5851
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-SP100 Antibody
Antibody SKU:CAB5851
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 181-480 of human SP100 (NP_001193631.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:MCF7, HeLa, 293T, HepG2, Mouse spleen, Mouse thymus, Mouse lung, Mouse heart, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 181-480 of human SP100 (NP_001193631.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FRSL TWPP SGSP SHAG TTPP ENGL SEHP CETE QINA KRKD TTSD KDDS LGSQ QTNE QCAQ KAEP TESC EQIA VQVN NGDA GREM PCPL PCDE ESPE AELH NHGI QINS CSVR LVDI KKEK PFSN SKVE CQAQ ARTH HNQA SDII VISS EDSE GSTD VDEP LEVF ISAP RSEP VINN DNPL ESND EKEG QEAT CSRP QIVP EPMD FRKL STFR ESFK KRVI GQDH DFSE SSEE EAPA EASS GALR SKHG EKAP MTSR STST WRIP SRKR RFSS SDFS DLSN GEEL QETC SSSL RRGS GKED
Gene ID:6672
Uniprot:P23497
Cellular Location:Cytoplasm, Nucleus, PML body
Calculated MW:50kDa/53kDa/78kDa/100kDa/101kDa
Observed MW:66-100kDa
Synonyms:SP100, lysp100b
Background:This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein.
UniProt Protein Function:Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2 according to PubMed:11909962. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA according to PubMed:15247905. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. Also plays a role in infection by viruses, including human cytomegalovirus and Epstein-Barr virus, through mechanisms that may involve chromatin and/or transcriptional regulation.
NCBI Summary:This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011]
UniProt Code:P23497
NCBI GenInfo Identifier:13878931
NCBI Gene ID:6672
NCBI Accession:P23497.3
UniProt Secondary Accession:P23497,O75450, Q13343, Q8TE34, Q96F70, Q96T24, Q96T95 B4DDX5, B8ZZD8, E7EUA7, E9PH61, F8WFE2,
UniProt Related Accession:P23497
Molecular Weight:50,032 Da
NCBI Full Name:Nuclear autoantigen Sp-100
NCBI Synonym Full Names:SP100 nuclear antigen
NCBI Official Symbol:SP100  
NCBI Official Synonym Symbols:lysp100b  
NCBI Protein Information:nuclear autoantigen Sp-100
UniProt Protein Name:Nuclear autoantigen Sp-100
UniProt Synonym Protein Names:Nuclear dot-associated Sp100 protein; Speckled 100 kDa
Protein Family:Nuclear autoantigen
UniProt Gene Name:SP100  
UniProt Entry Name:SP100_HUMAN
Anti-SP100 Antibody (CAB5851)Western blot analysis of extracts of various cell lines, using SP100 antibody (CAB5851) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
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