Cell Biology Antibodies 17
Anti-SP1 Antibody (CAB19649)
(No reviews yet)
Write a Review
- SKU:
- CAB19649
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SP1 Antibody |
| Antibody SKU: | CAB19649 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human SP1. |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, U-87MG, Rat liver |
| Immunogen: | A synthesized peptide derived from human SP1. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 6667 |
| Uniprot: | P08047 |
| Cellular Location: | |
| Calculated MW: | 90kDa |
| Observed MW: | 90KDa |
 | Western blot analysis of extracts of various cell lines, using SP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat spleen using SP1 antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using SP1 antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. |
 | Immunofluorescence analysis of C6 cells using SP1 antibody (A19649). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using SP1 antibody (A19649). Blue: DAPI for nuclear staining. |
 | Chromatin immunoprecipitation analysis of extracts of 293T cells, using SP1 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक
Human SP1 (Transcription factor Sp1) ELISA Kit (HUFI04863)
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीकी