KO Validated Antibodies 2

Anti-SOX2 Antibody [KO Validated] (CAB19118)

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SKU:
CAB19118
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-SOX2 Antibody [KO Validated]
Antibody SKU:CAB19118
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human SOX2
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, Rat brain, 293T
Immunogen:A synthesized peptide derived from human SOX2
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:6657
Uniprot:P48431
Cellular Location:
Calculated MW:34kDa
Observed MW:38kDa
Anti-SOX2 Antibody [KO Validated] (CAB19118)Western blot analysis of extracts of various cell lines, using SOX2 antibody (CAB19118) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-SOX2 Antibody [KO Validated] (CAB19118)Western blot analysis of extracts from normal (control) and SOX2 knockout (KO) 293T cells, using SOX2 antibody (CAB19118) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
Synonyms:ANOP3, MCOPS3, SOX2, SRY-box 2
Background:This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]
UniProt Protein Function:SOX2: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.
UniProt Protein Details:

Protein type:Cell development/differentiation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 3q26.33

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; transcription factor complex

Molecular Function:DNA binding; miRNA binding; protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: cell cycle arrest; endodermal cell fate specification; eye development; forebrain development; inner ear development; negative regulation of epithelial cell proliferation; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; osteoblast differentiation; pituitary gland development; positive regulation of MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of caspase activity; regulation of gene expression; regulation of transcription, DNA-dependent; response to wounding; somatic stem cell maintenance

Disease: Microphthalmia, Syndromic 3; Tracheoesophageal Fistula With Or Without Esophageal Atresia

NCBI Summary:This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]
UniProt Code:P48431
NCBI GenInfo Identifier:1351091
NCBI Gene ID:6657
NCBI Accession:P48431.1
UniProt Secondary Accession:P48431,Q14537,
UniProt Related Accession:P48431
Molecular Weight:34kDa
NCBI Full Name:Transcription factor SOX-2
NCBI Synonym Full Names:SRY-box 2
NCBI Official Symbol:SOX2  
NCBI Official Synonym Symbols:ANOP3; MCOPS3  
NCBI Protein Information:transcription factor SOX-2
UniProt Protein Name:Transcription factor SOX-2
UniProt Gene Name:SOX2  
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