Antibody Name:	Anti-SOX2 Antibody [KO Validated]
Antibody SKU:	CAB0561
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human SOX2 (NP_003097.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, MCF7, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human SOX2 (NP_003097.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MYNM METE LKPP GPQQ TSGG GGGN STAA AAGG NQKN SPDR VKRP MNAF MVWS RGQR RKMA QENP KMHN SEIS KRLG AEWK LLSE TEKR PFID EAKR LRAL HMKE HPDY KYRP RRKT KTLM KKDK YTLP GGLL APGG NSMA
Gene ID:	6657
Uniprot:	P48431
Cellular Location:	Nucleus
Calculated MW:	34kDa
Observed MW:	35KDa

Synonyms:

ANOP3, MCOPS3, SOX2, SRY-box 2

Background:

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

UniProt Protein Function:	SOX2: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.
UniProt Protein Details:	Protein type:Cell development/differentiation; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 3q26.33 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; transcription factor complex Molecular Function:DNA binding; miRNA binding; protein binding; sequence-specific DNA binding; transcription factor activity Biological Process: cell cycle arrest; endodermal cell fate specification; eye development; forebrain development; inner ear development; negative regulation of epithelial cell proliferation; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; osteoblast differentiation; pituitary gland development; positive regulation of MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of caspase activity; regulation of gene expression; regulation of transcription, DNA-dependent; response to wounding; somatic stem cell maintenance Disease: Microphthalmia, Syndromic 3; Tracheoesophageal Fistula With Or Without Esophageal Atresia
NCBI Summary:	This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]
UniProt Code:	P48431
NCBI GenInfo Identifier:	1351091
NCBI Gene ID:	6657
NCBI Accession:	P48431.1
UniProt Secondary Accession:	P48431,Q14537,
UniProt Related Accession:	P48431
Molecular Weight:	34kDa
NCBI Full Name:	Transcription factor SOX-2
NCBI Synonym Full Names:	SRY-box 2
NCBI Official Symbol:	SOX2
NCBI Official Synonym Symbols:	ANOP3; MCOPS3
NCBI Protein Information:	transcription factor SOX-2
UniProt Protein Name:	Transcription factor SOX-2
UniProt Gene Name:	SOX2

	Western blot analysis of extracts of MCF7 cells, using SOX2 antibody (CAB0561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of extracts of various cell lines, using SOX2 antibody (CAB0561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Western blot analysis of extracts from normal (control) and SOX2 knockout (KO) 293T cells, using SOX2 antibody (CAB0561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded mouse embryos using [KO Validated] SOX2 Rabbit pAb (CAB0561) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal using [KO Validated] SOX2 Rabbit pAb (CAB0561) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-SOX2 Antibody (CAB11501)	Human SOX2 ELISA Kit
Anti-SOX2 Antibody [KO Validated] (CAB19118)	Human Transcription factor SOX-2 (SOX2) ELISA Kit
SOX2 Antibody (PACO12400)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)