Antibody Name:	Anti-Sorting nexin-1 Antibody
Antibody SKU:	CAB8625
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SNX1 (NP_003090.2).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, LO2, A-549, HT-1080

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SNX1 (NP_003090.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASG GGGC SASE RLPP PFPG LEPE SEGA AGGS EPEA GDSD TEGE DIFT GAAV VSKH QSPK ITTS LLPI NNGS KENG IHEE QDQE PQDL FADA TVEL SLDS TQNN QKKV LAKT LISL PPQE ATNS SKPQ PTYE ELEE EEQE DQFD LTVG IT
Gene ID:	6642
Uniprot:	Q13596
Cellular Location:	Cell projection, Cytoplasmic side, Early endosome membrane, Endosome membrane, Golgi apparatus, Peripheral membrane protein, lamellipodium, trans-Golgi network membrane
Calculated MW:	51kDa/59kDa/63kDa
Observed MW:	80kDa

Synonyms:

SNX1, HsT17379, VPS5

Background:

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms.

UniProt Protein Function:	SNX1: May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3- phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Belongs to the sorting nexin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 15q22.31 Cellular Component: cytoplasm; early endosome membrane; endosome membrane; extrinsic to membrane; intracellular membrane-bound organelle; membrane; protein complex; retromer complex Molecular Function:epidermal growth factor receptor binding; identical protein binding; insulin receptor binding; phosphoinositide binding; protein binding; protein heterodimerization activity; protein homodimerization activity Biological Process: intracellular protein transport; receptor internalization; retrograde transport, endosome to Golgi; vesicle organization and biogenesis
NCBI Summary:	This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13596
NCBI GenInfo Identifier:	17380569
NCBI Gene ID:	6642
NCBI Accession:	Q13596.3
UniProt Secondary Accession:	Q13596,O60750, O60751, Q6ZRJ8, A6NM19, A8K6T7, H0Y2M5
UniProt Related Accession:	Q13596
Molecular Weight:	63,082 Da
NCBI Full Name:	Sorting nexin-1
NCBI Synonym Full Names:	sorting nexin 1
NCBI Official Symbol:	SNX1
NCBI Official Synonym Symbols:	VPS5; HsT17379
NCBI Protein Information:	sorting nexin-1
UniProt Protein Name:	Sorting nexin-1
Protein Family:	Sorting nexin
UniProt Gene Name:	SNX1
UniProt Entry Name:	SNX1_HUMAN

	Western blot analysis of extracts of various cell lines, using SNX1 antibody (CAB8625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of L929 cells using SNX1 Rabbit pAb (CAB8625) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using SNX1 Rabbit pAb (CAB8625) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.