Antibody Name:	Anti-SORT1 Antibody
Antibody SKU:	CAB7926
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 520-760 of human SORT1 (NP_002950.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	A-549, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 520-760 of human SORT1 (NP_002950.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EGPH YYTI LDSG GIIV AIEH SSRP INVI KFST DEGQ CWQT YTFT RDPI YFTG LASE PGAR SMNI SIWG FTES FLTS QWVS YTID FKDI LERN CEEK DYTI WLAH STDP EDYE DGCI LGYK EQFL RLRK SSVC QNGR DYVV TKQP SICL CSLE DFLC DFGY YRPE NDSK CVEQ PELK GHDL EFCL YGRE EHLT TNGY RKIP GDKC QGGV NPVR EVKD LKKK CTSN FLSP EKQN SKSN SVPI I
Gene ID:	6272
Uniprot:	Q99523
Cellular Location:	Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Extracellular side, Golgi apparatus, Golgi stack membrane, Lysosome membrane, Membrane, Nucleus membrane, Single-pass type I membrane protein
Calculated MW:	77kDa/92kDa
Observed MW:	100kDa

Synonyms:

SORT1, Gp95, LDLCQ6, NT3, NTR3, sortilin

Background:

This gene encodes a member of the VPS10-related sortilin family of proteins. The encoded preproprotein is proteolytically processed by furin to generate the mature receptor. This receptor plays a role in the trafficking of different proteins to either the cell surface, or subcellular compartments such as lysosomes and endosomes. Expression levels of this gene may influence the risk of myocardial infarction in human patients. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	SORT1: Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. A common polymorphism located in a non-coding region between CELSR2 and PSRC1 alters a CEBP transcription factor binding site and is responsible for changes in hepatic expression of SORT1. Altered SORT1 expression in liver affects low density lipoprotein cholesterol levels in plasma and is associated with susceptibility to myocardial infarction. Belongs to the VPS10-related sortilin family. SORT1 subfamily.
UniProt Protein Details:	Protein type:Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 1p13.3\|1p21.3-p13.1 Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; nuclear membrane; cell surface; clathrin-coated vesicle; lysosomal membrane; early endosome; cytoplasmic membrane-bound vesicle; integral to membrane; coated pit; trans-Golgi network transport vesicle; perinuclear region of cytoplasm; plasma membrane; endosome membrane Molecular Function:protein binding; nerve growth factor receptor activity; enzyme binding; nerve growth factor binding; neurotensin receptor activity, non-G-protein coupled Biological Process: ossification; nerve growth factor receptor signaling pathway; vesicle organization and biogenesis; multicellular organismal development; endosome transport via multivesicular body sorting pathway; myotube differentiation; Golgi to endosome transport; endocytosis; response to insulin stimulus; G-protein coupled receptor protein signaling pathway; induction of apoptosis via death domain receptors; neuropeptide signaling pathway; regulation of gene expression; glucose import; endosome to lysosome transport; plasma membrane to endosome transport; negative regulation of lipoprotein lipase activity Disease: Low Density Lipoprotein Cholesterol Level Quantitative Trait Locus 6
NCBI Summary:	This gene encodes a member of the VPS10-related sortilin family of proteins. The encoded preproprotein is proteolytically processed by furin to generate the mature receptor. This receptor plays a role in the trafficking of different proteins to either the cell surface, or subcellular compartments such as lysosomes and endosomes. Expression levels of this gene may influence the risk of myocardial infarction in human patients. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
UniProt Code:	Q99523
NCBI GenInfo Identifier:	84028263
NCBI Gene ID:	6272
NCBI Accession:	Q99523.3
UniProt Related Accession:	Q99523
Molecular Weight:	92kDa
NCBI Full Name:	Sortilin
NCBI Synonym Full Names:	sortilin 1
NCBI Official Symbol:	SORT1
NCBI Official Synonym Symbols:	NT3; Gp95; NTR3; LDLCQ6
NCBI Protein Information:	sortilin
UniProt Protein Name:	Sortilin
UniProt Synonym Protein Names:	100 kDa NT receptor; Glycoprotein 95; Gp95; Neurotensin receptor 3; NT3; NTR3
Protein Family:	Sortilin
UniProt Gene Name:	SORT1
UniProt Entry Name:	SORT_HUMAN

	Western blot analysis of extracts of various cell lines, using SORT1 antibody (CAB7926) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human kidney using SORT1 antibody (CAB7926) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using SORT1 antibody (CAB7926) at dilution of 1:100 (40x lens).