Cell Cycle Antibodies 1

Anti-SON Antibody (CAB16323)

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SKU:
CAB16323
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-SON Antibody
Antibody SKU:CAB16323
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 850-950 of human SON (NP_001278340.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:LO2, HeLa
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 850-950 of human SON (NP_001278340.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TMDS QMLA TSSM DSQM LASG TMDS QMLA SGTM DAQM LASG TMDA QMLA SSTQ DSAM LGSK SPDP YRLA QDPY RLAQ DPYR LGHD PYRL GHDA YRLG QDPY R
Gene ID:6651
Uniprot:P18583
Cellular Location:
Calculated MW:264kDa
Observed MW:250kDa
Synonyms:SON, BASS1, C21orf50, DBP-5, NREBP, SON3, TOKIMS
Background:This gene encodes a protein that contains multiple simple repeats. The encoded protein binds RNA and promotes pre-mRNA splicing, particularly of transcripts with poor splice sites. The protein also recognizes a specific DNA sequence found in the human hepatitis B virus (HBV) and represses HBV core promoter activity. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:SON: RNA-binding protein that acts as a mRNA splicing cofactor by promoting efficient splicing of transcripts that posses weak splice sites. Specifically promotes splicing of many cell-cycle and DNA-repair transcripts that posses weak splice sites, such as TUBG1, KATNB1, TUBGCP2, AURKB, PCNT, AKT1, RAD23A, and FANCG. Probably acts by facilitating the interaction between Serine/arginine-rich proteins such as SRSF2 and the RNA polymerase II. Also binds to DNA; binds to the consensus DNA sequence: 5'- GA[GT]AN[CG][AG]CC-3'. May indirectly repress hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. 10 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA-binding; DNA-binding

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: nuclear speck; nucleoplasm

Molecular Function:DNA binding; nucleic acid binding; protein binding; RNA binding; RS domain binding

Biological Process: cytokinesis; microtubule cytoskeleton organization and biogenesis; mRNA processing; negative regulation of apoptosis; regulation of cell cycle; regulation of RNA splicing; RNA splicing

NCBI Summary:This gene encodes a protein that contains multiple simple repeats. The encoded protein binds RNA and promotes pre-mRNA splicing, particularly of transcripts with poor splice sites. The protein also recognizes a specific DNA sequence found in the human hepatitis B virus (HBV) and represses HBV core promoter activity. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Code:P18583
NCBI GenInfo Identifier:296453022
NCBI Gene ID:6651
NCBI Accession:P18583.4
UniProt Secondary Accession:P18583,O14487, O95981, Q14120, Q6PKE0, Q9H7B1, D3DSF5 D3DSF6, E7ETE8, E7EU67, E7EVW3, E9PFQ2,
UniProt Related Accession:P18583
Molecular Weight:247,823 Da
NCBI Full Name:Protein SON
NCBI Synonym Full Names:SON DNA binding protein
NCBI Official Symbol:SON  
NCBI Official Synonym Symbols:SON3; BASS1; DBP-5; NREBP; C21orf50  
NCBI Protein Information:protein SON
UniProt Protein Name:Protein SON
UniProt Synonym Protein Names:Bax antagonist selected in saccharomyces 1; BASS1; Negative regulatory element-binding protein; NRE-binding protein; Protein DBP-5; SON3
UniProt Gene Name:SON  
UniProt Entry Name:SON_HUMAN
Anti-SON Antibody (CAB16323)Western blot analysis of extracts of various cell lines, using SON antibody (CAB16323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 8min.
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